文献类型：期刊文献

英文题名：Integrated Metabolomic and Transcriptomic Analysis Reveals Differential Mechanism of Flavonoid Biosynthesis in Two Cultivars of Angelica sinensis

作者：Zhu, Tiantian[1,2];Zhang, Minghui[1];Su, Hongyan[3];Li, Meiling[3];Wang, Yuanyuan[1,2];Jin, Ling[1,2];Li, Mengfei[3]

第一作者：朱田田;Zhu, Tiantian

通信作者：Jin, L[1];Jin, L[2];Li, MF[3]

机构：[1]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730101, Peoples R China;[2]Northwest Collaborat Innovat Ctr Tradit Chinese M, Lanzhou 730000, Peoples R China;[3]Gansu Agr Univ, State Key Lab Aridland Crop Sci, Lanzhou 730070, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730101, Peoples R China;[2]corresponding author), Northwest Collaborat Innovat Ctr Tradit Chinese M, Lanzhou 730000, Peoples R China;[3]corresponding author), Gansu Agr Univ, State Key Lab Aridland Crop Sci, Lanzhou 730070, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2022

卷号：27

期号：1

外文期刊名：MOLECULES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000743680900001)】；

基金：This research was funded by National Natural Science Foundation of China (81360615 and 32160083), Innovation Base and Talent Plan of Gansu Province of China (20JR5RA182), Double First-Class initiative projects of Gansu Province of China (GSSYLXM-05), Gansu University of Chinese Medicine (2021KCZD-4), Assurance Project of Ecological Planting and Quality of Daodi Herbs (202103003), and State Key Laboratory of Aridland Crop Science Gansu Agricultural University (GSCS-2021-Z03).

语种：英文

外文关键词：Angelica sinensis; cultivar; flavonoids; anthocyanins; metabolomics; transcriptomics

摘要：Angelica sinensis is a traditional Chinese medicinal plant that has been primarily used as a blood tonic. It largely relies on its bioactive metabolites, which include ferulic acid, volatile oils, polysaccharides and flavonoids. In order to improve the yield and quality of A. sinensis, the two cultivars Mingui 1 (M1), with a purple stem, and Mingui 2 (M2), with a green stem, have been selected in the field. Although a higher root yield and ferulic acid content in M1 than M2 has been observed, the differences of flavonoid biosynthesis and stem-color formation are still limited. In this study, the contents of flavonoids and anthocyanins were determined by spectrophotometer, the differences of flavonoids and transcripts in M1 and M2 were conducted by metabolomic and transcriptomic analysis, and the expression level of candidate genes was validated by qRT-PCR. The results showed that the contents of flavonoids and anthocyanins were 1.5- and 2.6-fold greater in M1 than M2, respectively. A total of 26 differentially accumulated flavonoids (DAFs) with 19 up-regulated (UR) and seven down-regulated (DR) were obtained from the 131 identified flavonoids (e.g., flavonols, flavonoid, isoflavones, and anthocyanins) in M1 vs. M2. A total 2210 differentially expressed genes (DEGs) were obtained from the 34,528 full-length isoforms in M1 vs. M2, and 29 DEGs with 24 UR and 5 DR were identified to be involved in flavonoid biosynthesis, with 25 genes (e.g., CHS1, CHI3, F3H, DFR, ANS, CYPs and UGTs) mapped on the flavonoid biosynthetic pathway and four genes (e.g., RL1, RL6, MYB90 and MYB114) belonging to transcription factors. The differential accumulation level of flavonoids is coherent with the expression level of candidate genes. Finally, the network of DAFs regulated by DEGs was proposed. These findings will provide references for flavonoid production and cultivars selection of A. sinensis.