文献类型：期刊文献

中文题名：党参治疗慢性阻塞性肺疾病的配伍用药规律及网络药理学研究

英文题名：Medication rules of compatibility and network pharmacology of Codonopsis radix in the treatment of chronic obstructive pulmonary disease

作者：郭鹏翔[1,2];王志旺[1];杨扶德[1];韦凌霞[1];丁茂鹏[1];冯金梁[3]

第一作者：郭鹏翔

机构：[1]甘肃中医药大学药学院,甘肃兰州730101;[2]甘肃中医药大学附属医院药学部,甘肃兰州730020;[3]甘肃康乐药业有限责任公司质检室,甘肃兰州730300

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2021

卷号：38

期号：4

起止页码：66

中文期刊名：甘肃中医药大学学报

外文期刊名：Journal of Gansu University of Chinese Medicine

基金：科技部国家重点研发计划项目(2018YFC1706305);甘肃省科技小巨人企业培育计划项目(17CXIJA084);2018年度甘肃省委组织部人才发展专项资金项目(甘组[2018]23号);2018年兰州市人才创新创业科技计划项目(2017-RC-112);中科院兰州化物所"十三五"重大突破项目(2016-2020)。

语种：中文

中文关键词：慢性阻塞性肺疾病;党参;网络药理学;作用机制

外文关键词：chronic obstructive pulmonary disease(COPD);Codonopsis radix;network pharmacology;action mechanism

摘要：目的基于数据挖掘和网络药理学方法分析党参治疗慢性阻塞性肺疾病(COPD)的用药规律及其作用机制。方法收集中国知网(CNKI)中党参治疗COPD的所有方剂,对符合纳入标准的中药的频次、性味归经及功效进行分类统计,并将频数≥50的中药进行关联规则分析;采用中药系统药理学数据库与分析平台(TCMSP)筛选党参的活性成分及作用靶点;使用疗效药靶数据库(TTD)、美国国家生物技术信息中心(NCBI)gene数据库、Dis Ge NET数据库获取COPD的相关靶点,二者匹配获得党参治疗COPD的关键靶点;利用STRING数据库进行蛋白质-蛋白质相互作用(PPI)分析,采用R语言Cluster Profiler包进行基因本体(GO)功能和京都基因与基因组百科全书(KEGG)通路富集分析;最后借助Cytoscape 3.4.0软件构建成分-靶点、通路-靶点及PPI网络图,计算网络拓扑参数。结果收集党参治疗COPD的复方共计737首,涉及中药294味,以补益药、化痰止咳平喘药多见,温、平、寒居多,甘、苦、辛为主,主要归肺、脾经;收集党参活性成分21个,靶点101个,与884个COPD靶点匹配,获取关键靶点38个,通过相关软件分析核心成分有豆甾醇(Stigmasterol)、黄豆黄素(Glycitein)及木犀草素(Luteolin)等,核心靶点有白细胞介素(IL)-6、前列腺素内过氧化物合酶2(PTGS2)及血管内皮生长因子A(VEGFA)等,KEGG通路富集显示,党参主要通过IL-17、肿瘤坏死因子-α(TNF-α)及磷脂酰肌醇3-激酶-蛋白激酶B(PI3K-Akt)等信号通路发挥治疗COPD的作用。结论党参常配伍补虚类等药物治疗COPD,其主要通过"多成分-多靶点-多通路"的分子机制发挥治疗COPD的作用。
Objective To analyze the medication rules and action mechanism of Codonopsis radix in the treatment of chronic obstructive pulmonary disease( COPD) based on data mining and network pharmacology. Methods All the prescriptions of Codonopsis radix in the treatment of COPD from China National Knowledge Infrastructure( CNKI) were collected,and the frequency,property,flavour and meridian tropism along with efficacy of Chinese materia medica( CMM) met the inclusion criteria were classified statistically,and the CMM with frequency more than 50 was analyzed by association rules. The active components of Codonopsis radix and its targets were screened out by searching Traditional Chinese Medicine Systems Pharmacology( TCMSP) Database and Analysis platform;Therapeutic Target Database( TTD),National Center for Biotechnology Information( NCBI) gene database and DisGeNET database were used to obtain the relevant targets of COPD,and the key targets of Codonopsis radix in the treatment of COPD were obtained by matching the two databases. Search Tool for the Retrieval of Interacting Proteins( STRING) database was used to network analyze protein-protein interaction( PPI),gene ontology( GO) function enrichment and Kyoto encyclopedia of genes and genomes( KEGG) signal pathway enrichment analysis were performed on R language Cluster Profiler packets. Finally,the component-target and pathway-target and PPI network graphs were constructed with Cytoscape 3. 4. 0 software,and network topological parameters were calculated.Results A total of 737 of complex prescriptions of Codonopsis radix in the treatment of COPD were collected,involving 294 flavors of CMM,it was common to see tonics and medicinals with resolving phlegm,stopping cough and relieving asthma,of which those with warm,mild and cold property were in the majority,and those with sweet,bitter and pungent flavors are in the main,and the main meridian distribution were lung and spleen meridians. Twenty-one active components and 101 targets of Codonopsis radix were collected,and 38 key targets were obtained by matching with 884 COPD targets. Through the analysis of related software,the core components of Codonopsis radix were stigmasterol,glycitein and luteolin etc.,and the core targets were interleukin( IL)-6,prostaglandin endope-roxide synthase 2( PTGS2) and vascular endothelial growth factor A( VEGFA) etc.,KEGG pathway enrichment showed that Codonopsis radix played a role in the treatment of COPD mainly through IL-17,tumor necrosis factor-α( TNF-α)and PI3 K-Akt signaling pathways.Conclusion Codonopsis radix often combines with reinforcing deficiency type of medicinals to treat COPD,which plays the role of treating COPD mainly via "multi-component,multi-target and multi-pathway"molecular mechanism.