文献类型：期刊文献

中文题名：基于HPLC指纹图谱及色度值的当归与油当归比较研究

英文题名：Comparative Study on the Difference between Angelicae Sinensis Radix and Oil Angelicae Sinensis Radix Based on HPLC Fingerprint and Chromaticity Value

作者：党文飞[1];张红伟[1];周洁[1];周晶晶[1];窦霞[2,3];靳子明[2,3]

第一作者：党文飞

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]甘肃中医药大学附属医院,甘肃兰州730000;[3]杨锡仓全国名老中医药专家工作室,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2024

卷号：31

期号：5

起止页码：112

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2023_2024】；

基金：甘肃省中药炮制技术传承基地项目(2022年);甘肃省自然科学基金(21JR7RA574);甘肃省中医药科研课题(GZKP-2022-23);兰州市人才创新创业项目(2021-RC-104);兰州市城关区科技计划(2021-2-9)。

语种：中文

中文关键词：当归;油当归;指纹图谱;色度值;多元统计分析

外文关键词：Angelicae Sinensis Radix;oil Angelicae Sinensis Radix;fingerprint;chromaticity value;multivariate statistical analysis

摘要：目的通过建立指纹图谱结合色度值分析当归与油当归的差异。方法采用HPLC建立当归油炒前后样品指纹图谱,评价相似度并指认共有峰;以当归油炒前后共有峰峰面积、色度值为指标,运用配对样本t检验、聚类热图分析、主成分分析和正交偏最小二乘-判别分析(OPLS-DA)对当归油炒前后样品进行区分,以变量重要性投影(VIP)>1为标准筛选差异性标志物。结果当归、油当归指纹图谱分别标定16、18个共有峰,其中色谱峰8、10号为炮制后产生,共指认6个成分,分别为阿魏酸、绿原酸、藁本内酯、洋川芎内酯I、洋川芎内酯H、阿魏酸松柏酯。聚类热图、主成分分析结果与OPLS-DA结果一致,均可将样品明显聚为当归和油当归2类;通过VIP值筛选出峰5、峰7(绿原酸)、峰17、峰4、峰18(藁本内酯)、峰9(阿魏酸)、峰1、峰13、峰11(洋川芎内酯I)、峰2、峰3为导致样品差异的主要标志色谱峰。油当归与当归的色度值差ΔE*值为6.10~12.37,表明二者可被肉眼识别,且L*、a*、b*均可作为鉴别当归与油当归的关键指标。结论建立的当归和油当归HPLC指纹图谱方法稳定可靠,结合色度值差异可用于区分当归与油当归。
Objective To analyze the difference between Angelicae Sinensis Radix and oil Angelicae Sinensis Radix by establishing a fingerprint and combining the chromaticity value.Methods HPLC method was used to establish the fingerprint before and after frying Angelicae Sinensis Radix with oil,and the similarity was evaluated and the common peak was identified.Taking the total peak area and chromaticity value before and after frying Angelicae Sinensis Radix with oil as the indicators,paired sample t-test,cluster heat map analysis,principal component analysis and orthogonal partial least squares-discriminant analysis(OPLS-DA)were used to distinguish before and after frying Angelicae Sinensis Radix with oil.The differential markers were screened out by variable importance projection(VIP)>1.Results The established fingerprints of Angelicae Sinensis Radix and oil Angelicae Sinensis Radix were calibrated with 16 and 18 common peaks,respectively,among which,chromatographic peaks 8 and 10 were generated after processing.A total of 6 components were identified,including ferulic acid,chlorogenic acid,ligustilide,senkyunolide I,senkyunolide H,and ferulic acid pine ester.The clustering heatmap and principal component analysis results were consistent with the OPLS-DA results,and the samples could be clearly clustered into two categories:Angelicae Sinensis Radix and oil Angelicae Sinensis Radix.Through VIP value screening,peaks 5,7(chlorogenic acid),17,4,18(ligustilide),9(ferulic acid),1,13,11(senkyunolide I),2,and 3 were identified as the main chromatographic peaks that caused sample differences.The difference in chromaticity valueΔE*between Angelicae Sinensis Radix and oil Angelicae Sinensis Radix was 6.10 to 12.37,indicating that they could be recognized by the naked eye,and L*,a*and b*could all be used as key indicators for distinguishing Angelicae Sinensis Radix from oil Angelicae Sinensis Radix.Conclusion The established HPLC fingerprinting method for Angelicae Sinensis Radix and oil Angelicae Sinensis Radix is stable and reliable,and can be used to distinguish Angelicae Sinensis Radix from oil Angelicae Sinensis Radix by combining the difference in chromaticity value.