文献类型：期刊文献

中文题名：槐角不同炮制品中槐角苷的含量测定

英文题名：Determination of sophoricoside in different processed products of sophora fruit

作者：金月[1];谢静[2];张岩[1];赵海鹰[1];马伟丽[1];常承东[1]

第一作者：金月

机构：[1]甘肃中医药大学附属医院制剂中心,甘肃兰州730000;[2]甘肃省嘉峪关市食品药品和医疗器械检验检测中心中药科,甘肃嘉峪关735300

第一机构：甘肃中医药大学第二附属医院

年份：2022

卷号：12

期号：5

起止页码：75

中文期刊名：中国医药科学

外文期刊名：China Medicine And Pharmacy

基金：甘肃省中医药管理局科研课题(GZK-2018-12)。

语种：中文

中文关键词：槐角;砂炒槐角;麸炒槐角;槐角苷;含量测定

外文关键词：Sophora fruit;Sand-fried sophora fruit;Bran-fried sophora fruit;Sophoricoside;Content determination

摘要：目的比较槐角不同炮制品中槐角苷成分的含量。方法采用HPLC法测定槐角苷的含量:Inertsil ODS C_(18)色谱柱(250 mm×4.6 mm,5μm);柱温30℃;流动相为甲醇-乙腈-0.1磷酸溶液(12∶20∶68);等度洗脱;体积流量1.0 ml/min;检测波长:260 nm。比较槐角生品、砂炒、麸炒炮制品槐角苷的含量。结果不同槐角炮制品中槐角苷的含量:生品(6.15%)>砂炒(5.92%)>麸炒(5.74%)。结论槐角饮片中黄酮类成分经过砂炒、麸炒炮制后槐角苷含量略降低。
Objective To compare the content of s ophoricoside in different proces sed products of sophora fruit.Methods The content of sophoricoside was determined by High Performance Liquid Chromatography(HPLC)in an Inertsil ODS C_(18) column(250 mm×4.6 mm,5μm)with column temperature of 30℃.The isocratic elution was performed with a mobile phase of methanol-acetonitrile-0.1 phosphoric acid solution(12∶20∶68).The volume flow rate was set to 1.0 ml/min,and the detection wavelength was 260 nm.The contents of sophoricoside in sophora fruit of raw,sand-fried and bran-fried were compared.Results The content of sophoricoside in different processed products of sophora fruit:raw sophora fruit(6.15%)>sand-fried sophora fruit(5.92%)>bran-fried sophora fruit(5.74%).Conclusion The content of sophoricoside in sophora fruit decoction pieces is slightly reduced after the flavonoids contained are s and-fried and bran-fried.