文献类型：期刊文献

中文题名：基于特征图谱及多指标综合评价的黄白益肺饮纯化工艺研究

英文题名：Research on the Purification Process of Huangbaiyi Lung Drink Based on Characteristic Map and Multi-Index Comprehensive Evaluation

作者：王博[1];黄雪艳[1];刘晓霞[1];冯晓莉[1];武晓玉[1];侯昕阳[1];魏舒畅[1,2,3]

第一作者：王博

机构：[1]甘肃中医药大学,兰州730000;[2]甘肃省中药制药工艺工程研究中心,兰州730000;[3]陇药产业创新研究院,兰州730000

第一机构：甘肃中医药大学

年份：2025

卷号：60

期号：7

起止页码：739

中文期刊名：中国药学杂志

外文期刊名：Chinese Pharmaceutical Journal

收录：;北大核心:【北大核心2023】；

基金：甘肃省高等学校产业支撑引导项目资助(2019C-02)。

语种：中文

中文关键词：黄白益肺饮;特征图谱;化学模式识别;层次分析法;超滤纯化

外文关键词：Huangbaiyi Lung Drink;feature map;chemical pattern recognition;analytic hierarchy process;ultrafiltration purification

摘要：目的建立黄白益肺饮的特征图谱及多成分含量测定方法,并用陶瓷膜超滤技术对提取液的纯化工艺进行研究,建立一种适合于工业生产的纯化工艺。方法用HPLC法建立黄白益肺饮的特征图谱并结合化学模式识别筛选差异性成分,并采用正交设计试验L_(9)(3^(4)),以被指认出的成分、总多糖、醇浸出物的保留率及除杂率为工艺评价指标,优化黄白益肺饮水提液的最佳超滤工艺参数。结果所建立的特征图谱有19个共有峰,并指认出绿原酸等4个成分,筛选出毛蕊异黄酮葡萄糖苷等11个差异性成分。最佳超滤工艺条件为膜孔径20 nm、操作压力0.12 MPa、料液温度20℃、膜面错流速度4.6 m·s^(-1),总多糖、乙醇浸出物、绿原酸、毛蕊异黄酮葡萄糖苷、甘草苷、芦丁保留率分别为82.86%、84.23%、92.97%、92.54%、92.92%、92.29%,除杂率可达11.73%。结论所建立的多成分含量测定方法可靠并且筛选出的超滤精制工艺生产成本低、安全性好、重复性高,有良好的工业化生产前景,可为中药复方水提液的精制提供参考。
OBJECTIVE To establish the characteristic map and multi-component content determination method of Huangbaiyi Lung Drink,and to study the purification process of the extract by ceramic membrane ultrafiltration technology,and to establish a purification and refining method for the extraction of Huangbaiyi Lung Drinking Water suitable for industrial production.METHODS HPLC was used to establish the characteristic map of Huangbaiyi Lung Drink,combined with chemical pattern recognition to screen the differential components,and the orthogonal design experiment L_(9)(3^(4))was used to optimize the optimal ultrafiltration process parameters of Huangbaiyi Lung Drinking Water extract with the identification components,the retention rate of total polysaccharides and alcohol extracts and the impurity removal rate were the process evaluation indexes.RESULTS There were 19 common peaks in the characteristic map,4 components such as chlorogenic acid,and 11 differential components such as verbascoside isoflavone glucoside were screened.The optimal ultrafiltration process conditions were as follows:membrane pore size of 20 nm,operating pressure of 0.12 MPa,feed liquid temperature of 20℃,cross-flow velocity of membrane surface of 4.6 m·s^(-1),the retention rates of total polysaccharides,ethanol extracts,chlorogenic acid,verbasyl isoflavone glucoside,glycyrrhizin and rutin were 82.86%,84.23%,92.97%,92.54%,92.92%and 92.29%,respectively,and the impurity removal rate was 11.73%.CONCLUSION The established method for the determination of multi-component content is reliable,and the selected ultrafiltration refining process has low production cost,good safety and high repeatability,and has a good prospect for industrial production,which can provide a reference for the refining of aqueous extract of traditional Chinese medicine compounds.