文献类型：期刊文献

中文题名：秦艽醇提物对胶原诱导性关节炎大鼠血清抗-CCP及TNF-α的影响

英文题名：Effect of Alcohol Extractive from Gentianae Macrophyllae Radix on Serum Anti-CCP and TNF-α in Collagen Induced Arthritis Rats

作者：王钢[1];王丽琴[2];王佳[1];王涛[1];刘卓[2]

第一作者：王钢

机构：[1]甘肃中医学院附属医院风湿骨病科,兰州730000;[2]甘肃中医学院,兰州730000

第一机构：甘肃中医药大学第二附属医院

年份：2013

卷号：19

期号：19

起止页码：302

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2011】；

基金：甘肃省中医药管理局基金项目(GZK-2010-3)

语种：中文

中文关键词：大叶秦艽醇提物;胶原诱导性关节炎大鼠;抗-CCP抗体;肿瘤坏死因子-α

外文关键词：big leaf Gentianae Macrophyllae Radix alcohol extract;;collagen induced arthritis rats;;anticyclic citrullinater peptide antibody;;tumor necrosis factor-α

摘要：目的:研究大叶秦艽醇提取物对胶原诱导性关节炎大鼠的防治作用,并初步探讨其作用机制。方法:随机将96只大鼠分为正常组、模型组、白芍组及秦艽组。除正常组外,余各组用胶原乳液多点皮内注射建立关节炎模型。模型建立后第9天开始秦艽组以秦艽乙醇提物900 mg·kg-1·d-1灌胃,白芍组给予白芍总苷胶囊内容物108 mg·kg-1·d-1灌胃,模型组给予蒸馏水0.5 mL·kg-1·d-1灌胃。造模后14,21,28 d观察各组大鼠一般情况、关节肿胀程度、ELISA法检测血清抗环瓜氨酸肽(CCP)抗体及肿瘤坏死因子-α(TNF-α)水平。结果:模型组大鼠的踝关节及足趾关节肿胀明显且血清中抗-CCP抗体及TNF-α水平明显升高,秦艽组及白芍组与模型组比较关节肿胀程度明显减轻,血清中抗-CCP抗体及TNF-α水平明显降低,有统计学意义(P<0.05);免疫后28 d秦艽组与白芍组比较,大鼠关节肿胀程度明显减轻且TNF-α表达降低明显有统计学意义(P<0.05)。结论:大叶秦艽醇提物能够在早期抑制胶原诱导性关节炎大鼠血清中抗-CCP抗体及TNF-α水平,减轻关节肿胀程度,改善滑膜炎症状,保护关节,体现治疗早期类风湿关节炎(RA)的优势。
Objective: To study the effect,of big leaf Gentianae Macrophyllae Radix alcohol extract on serum anti-cyclic citrullinafer peptide( CCP) and tumor necrosis factor-α( TNF-α) in collagen induced arthritis rat control. Method: Ninty-six rats were randomLy divided into: normal group,model group,Paeoniae Alba Radix and Gentianae Macrophyllae Radix group group. In addition to the normal group,each group was given collagen emulsion multipoint to establish arthritis model. After modelling,corresponding drugs was given on day14,21 and 28,the general status,and joint swelling degree were observed. ELISA method was used to detect serum anti-CCP antibody and TNF-α level. Result: In model rats,ankle and digits joint swelling was obvious and serum anti-CCP antibody and TNF-α level obviously increased,in Gentianae Macrophyllae Radix group and radix paeoniae alba group,compared with model group ankle joint swelling and toes significantly reduced and serum anti-CCP antibody and TNF-α level decreased obviously,there was a statistical significance( P < 0. 05); after 28days in Gentianae Macrophyllae Radix group compared with Paeoniae Alba Radix group,joint swelling of rats significantly reduced and TNF-α reduced obviously( P < 0. 05). Conclusion: The big leaf Gentianae Macrophyllae Radix alcohol extract can decrease in collagen induced arthritis rats serum anti-CCP antibody and TNF-α level,reduce joint swelling degree,and improve the synovial inflammation shape.