文献类型：期刊文献

英文题名：Protective Activities of Cistanoside A on CCl₄ Induced Hepatotoxicity in Mice

作者：Luo, Huiying[1,2];Li, Juan[3];Wang, Lijuan[1,2];Zhu, Lijuan[1,2]

第一作者：Luo, Huiying;罗慧英

通信作者：Luo, HY[1]

机构：[1]Gansu Coll Tradit Chinese Med, Dept Pharmacol, Lanzhou 730000, Peoples R China;[2]Key Lab Pharmacol & Toxicol Tradit Chinese Med Ga, Lanzhou 730000, Peoples R China;[3]Lanzhou Univ, Hosp 1, Ctr Mol Biol, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Coll Tradit Chinese Med, Dept Pharmacol, Av Dingxi 30, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2012

卷号：31

期号：3

起止页码：407

外文期刊名：LATIN AMERICAN JOURNAL OF PHARMACY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000305594500009)】；

语种：英文

外文关键词：Cistanoside A; CCl4; Free radical; Hepatoprotection; Respiratory chain

摘要：To evaluate the protective efficacy of Cistanoside A (C.A), a phenylethanol glycoside isolated from Cistanche deserticola, on CCl4 induced hepatotoxicity in mice, 50 animals were divided into five different protocols, and hepatic functional index were detected by diagnostic kits. Histological changes were compared by H&E stain. Activities of mitochondrial antioxidant enzymes (GST, SOD, and CAT) and respiratory marker enzymes (MDH, SDH, NADH dehydrogenase, and cytochrome c oxidases) were measured. To confirm the effect of C.A on free radical, tests on the free radical scavenging activities were also carried out in vitro. We found treatment with C.A (10, 20 mg/kg o.p. for 7 days) could significantly ameliorated the levels of hepatic function indices (AST, ALT, ALP and LDH) (P < 0.05). The biochemical results were also confirmed by histopathological examination. C.A treatment decreased the ballooning degeneration, moderated the hepatocytes apoptosis, and alleviated centrilobular and bridging necrosis which were observed in the CCl4 control group. Following experiments revealed that C.A could increase the activities of mitochondrial antioxidant enzymes (GST, SOD, and CAT) and respiratory marker enzymes (MDH, SDH, NADH dehydrogenase, and cytochrome c oxidases) (P < 0.05). In vitro, C.A exhibited strong scavenging activities for DPPH radical and superoxide anion radical. Our results revealed that C.A possess protective activities on CCl4 induced hepatotoxicity in mice, which was involved with increasing free radicals clearing activities, alleviating lipid-overoxidation damage, and improving respiratory chain function in mitochondria.