文献类型：期刊文献

中文题名：改进差示苯酚-硫酸法测定维血宁颗粒多糖含量

英文题名：Determination of Polysaccharides in Weixuening Granule by Improved Differential Phenol-sulfuric Method

作者：陈晖[1];闫治攀[1];陈方圆[1];王继龙[1];魏舒畅[1];王碧辉[2]

第一作者：陈晖

机构：[1]甘肃中医学院,甘肃兰州730000;[2]兰州太保制药有限公司,甘肃兰州730050

第一机构：甘肃中医药大学

年份：2014

卷号：21

期号：3

起止页码：84

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2013_2014】；

基金：甘肃省科技重大专项计划项目(1002FKDA051)

语种：中文

中文关键词：维血宁颗粒;苯酚-硫酸法;多糖;含量测定

外文关键词：Weixuening Granule;phenol-sulfuric method;polysaccharides;content determination

摘要：目的：建立维血宁颗粒中多糖含量测定方法,以更好地控制该制剂质量。方法采用改进差示苯酚-硫酸法,以葡萄糖为对照品,在波长487 nm处,以苯酚浓度、浓硫酸浓度和反应时间为考察因素,优选最佳显色条件。结果测定多糖的最佳显色条件为0.04 g/mL苯酚溶液1 mL、87.5%硫酸溶液5 mL和显色时间为15 min；葡萄糖在15.3～306.0μg范围内与吸光度差值（ΔA）呈良好的线性关系,r＝0.9997；平均回收率为103.5%, RSD＝2.9%。测得维血宁颗粒中多糖平均含量为27.04%,RSD＝1.1%。结论该方法操作简便、准确,稳定可靠,可用于维血宁颗粒中多糖含量的测定。
Objective To establish a method for determination of polysaccharides in Weixuening Granule, which can be used for quality control of the preparation. Methods Polysaccharides was determined by improved differential phenol-sulfuric method with glucose as reference, and the color conditions were optimized at the wavelength of 487 nm, with the concentration of phenol and sulfuric acid, and reaction time as investigated factors. Results The best color conditions of polysaccharides were 0.04 g/mL phenol 1 mL, 87.5% sulfuric acid 5 mL, and reaction time of 15 min. Glucose had a good linear relationship with absorbanceΔA at the range of 15.3-306.0μg, r=0.999 7. The average recovery was 103.5%, and RSD was 2.9%. The average content of polysaccharides in Weixuening Granule was 27.04%and RSD was 1.1%. Conclusion The method is simple, accurate, stable and reliable, and can be used to determine the content of polysaccharides in Weixuening Granule.