文献类型：期刊文献

中文题名：久泻灵冲剂对溃疡性结肠炎大鼠结肠病变氧化损伤的影响

英文题名：Effects of Jiuxieling Granules on Inflammatory Lesion of Colon Mucous Membrane in Rats with Ulcerative Colitis

作者：李海龙[1];吴玉泓[1];楚惠媛[1];车敏[1];安方玉[1];王宏伟[2]

第一作者：李海龙

机构：[1]甘肃中医学院,甘肃兰州730050;[2]甘肃中医学院附属医院,甘肃兰州730050

第一机构：甘肃中医药大学

年份：2009

卷号：16

期号：9

起止页码：29

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2011_2012】；

基金：兰州市科技局资助(2008-1-64)

语种：中文

中文关键词：久泻灵冲剂;溃疡性结肠炎;谷胱甘肽过氧化物酶;丙二醛;超氧化物歧化酶;一氧化氮;一氧化氮合酶;大鼠

外文关键词：Jiuxielinggranules; ulcerative colitis; GSH-Px; MDA; SOD; NO; NOS; rat

摘要：目的观察久泻灵冲剂对溃疡性结肠炎模型大鼠的影响,探讨其作用机制。方法采用异种异体结肠黏膜组织致敏法和乙酸局部灌肠相结合的方法制造动物模型。将Wistar大鼠随机分为空白对照组、模型组、阳性对照组及久泻灵冲剂大、中、小剂量组。连续给药14d,末次给药后24h处死大鼠,制备结肠组织匀浆,生化法检测结肠组织中谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、超氧化物歧化酶(SOD)、一氧化氮(NO)、总一氧化氮合酶(tNOS)和诱导型一氧化氮合酶(iNOS)的水平。结果久泻灵冲剂可明显降低结肠组织中NO、tNOS、iNOS和MDA的活性或水平,提高GSH-Px、SOD的活性。结论久泻灵冲剂对溃疡性结肠炎模型大鼠有显著的干预作用,其机制可能与抗氧自由基损伤,抑制NO、tNOS和iNOS生成,增强抗氧化酶活性有关。
Objective To observe the effects of Jiuxieling granules on rats with ulcerative colitis and explore its mechanism. Methods Ulcerative colitis models were established by immune method combined with local stimulation of 5% acetic acid. Wistar rats were randomly divided into control, model, positive control and low, middle, high dose Jiuxieling granules group. Rats in positive control group were treated with sulfasalazine, and those in three treatment groups were treated with low, middle and high dosage of Jiuxieling granules respectively for 14 days continuously. 24 hours after last administration, the rats were killed to obtain colon mucous membrane to test GSH-Px, SOD, MDA, NO, tNOS and iNOS concentration of the homogenate of colon mucous membrane. Results Jiuxieling granules could markedly improve,the levels of GSH-Px and SOD activity, whereas decrease the levels of MDA, NO, TNOS and iNOS. Conclusion Jiuxieling granules exert its marked curative effects on rats of ulcerative colonitis by resisting oxygen free radical lesion, inhibiting the generation of NO and NOS, and enhancing the activity of antioxidase.