文献类型：期刊文献

中文题名：正交试验优化大鼠糖尿病胃轻瘫模型

英文题名：Optimization of diabetic gastroparesis rat model by orthogonal design

作者：吴国泰[1,2];杜丽东[1,2];景琪[1];高云娟[1];刘五州[1];李应东[2];任远[1,2]

第一作者：吴国泰

机构：[1]甘肃中医学院药理学教研室,甘肃兰州730000;[2]甘肃省中药药理与毒理学重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2014

卷号：45

期号：14

起止页码：2047

中文期刊名：中草药

外文期刊名：Chinese Traditional and Herbal Drugs

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金："十二五"国家科技支撑计划项目子课题(2011BAI05B0206);甘肃省自然科学基金项目(1212RJZA079);甘肃省中药药理与毒理学重点实验室开放基金项目(ZDSYS-KJ-2012-004)

语种：中文

中文关键词：四氧嘧啶;糖尿病胃轻瘫;正交试验;胃动素;动物模型

外文关键词：alloxan; diabetic gastroparesis; orthogonal test; motilin; model

摘要：目的通过正交试验优化糖尿病胃轻瘫大鼠模型的制备方法。方法采用四氧嘧啶(ALX)法复制大鼠糖尿病胃轻瘫模型;采用4因素3水平的正交试验设计,以空腹血糖、胃内残留率和血浆胃动素(MOT)水平综合评分为评价指标,观察不同影响因素对大鼠糖尿病胃轻瘫模型形成的影响。结果饲料类型对大鼠糖尿病胃轻瘫模型的影响不明显,造模时间、ALX剂量对大鼠糖尿病胃轻瘫的形成有决定性影响,3个影响因素重要性依次为造模时间>ALX剂量>饲料类型。结论通过优化试验方法,可有效提高糖尿病胃轻瘫大鼠模型的造模效果,为科学评价糖尿病胃轻瘫的药物研究提供技术平台。
Objective To optimizethepreparation methods of diabetic gastroparesis rat model by orthogonal test. Methods The diabetes gastroparesis rat model was duplicated with alloxan （ALX） by using four factors and three levels of orthogonal design. The fasting blood glucose, gastric emptying rate, and plasma motilin level were scored as evaluation indexes, and the influence of different factors on diabetic gastroparesis model rats was observed. Results The effect of feed type on the diabetic gastroparesis rat model was not obvious, but the effects of modeling time and modeling agent ALX dose on diabetic gastroparesis rat model were decisive. The order of the importance was as follows： modeling time 〉 dose ofALX 〉 feed types. Conclusion The optimized test method can effectively improve the diabetic gastroparesis rat model and provide a technical platform for the study on drug evaluation of diabetic gastroparesis.