文献类型：期刊文献

中文题名：铁棒锤种子中乌头碱的提取方法优化及HPLC含量测定

英文题名：Extraction optimization and determination of aconitine in the seed of Aconitum pendulum Busch by HPLC

作者：林丽[1,2];杨建成[3];晋玲[1,2];王耀鹏[3];高素芳[1,2]

第一作者：林丽

机构：[1]甘肃中医学院,甘肃兰州730000;[2]甘肃省高校中(藏)药化学与质量研究省级重点实验室,甘肃兰州730000;[3]甘肃中医学院药学系,2010药物制剂甘肃兰州730000

第一机构：甘肃中医药大学

年份：2015

卷号：34

期号：3

起止页码：31

中文期刊名：中兽医医药杂志

外文期刊名：Journal of Traditional Chinese Veterinary Medicine

基金：甘肃教育厅科研项目(0906B07);甘肃中医学院中青年科研基金项目(09ZQ17;1106B-02)

语种：中文

中文关键词：HPLC;铁棒锤种子;提取方法;乌头碱

外文关键词：HPLC;;Aconitum pendulum Busch;;extracting methods;;aconitine

摘要：目的:优选铁棒锤种子中乌头碱的最佳提取方法。方法:分别采用浸泡法、超声提取法和回流法提取铁棒锤种子中乌头碱,采用HPLC方法测定其含量,色谱条件:ZORBAX Edipse XDB-C18色谱柱(4.6 mm×250 mm,5μm);G131513二极管阵列检测器;柱温30℃;流动相为乙腈-0.3%二乙胺水溶液(50∶50);流速1.0 m L/min;检测波长240 nm。比较分析测定结果,优选出最佳提取方法。结果:测得以浸泡法提取乌头碱的含量最高,为0.15%;超声提取法次之,为0.10%;回流法提取效果较差,为0.039%。结论:优选出铁棒锤种子中乌头碱的最佳提取方法为浸泡提取法,简便易行,重现性好。
Objective: To optimize the extraction technology for aconitine in the seed of Aconitum pendulum Busch. Methods:Aconitine was extracted from the seed of Aconitum pendulum Busch by immersion extraction, ultrasonic extraction and reflux method, respectively. Then the content of aconitine was determined by HPLC, and the extraction technology was optimized through the comparison and analysis of the results. Results: The content of aconitine was up to 0.15% by immersion extraction,then was ultrasonic extraction of 0.10%, and reflux method was the lowest of 0.039%. Conclusion: The best method was immersion extraction for extracting aconitine from the seed of Aconitum pendulum Busch.