文献类型：期刊文献

英文题名：Turn-on fluorescence detection of carbon monoxide in plant tissues based on Cu²⁺ modulated polydihydroxyphenylalanine nanosensors

作者：Mu, Xiqiong[1,2];Wang, Yinquan[2];Xu, Jian[1];Zeng, Fankui[1,3,4]

第一作者：Mu, Xiqiong

通信作者：Xu, J[1];Zeng, FK[1];Zeng, FK[2];Zeng, FK[3]

机构：[1]Chinese Acad Sci, Lanzhou Inst Chem Phys, Res Ctr Nat Med & Chem Metrol, Lanzhou 730000, Peoples R China;[2]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730101, Peoples R China;[3]Yantai Zhongke Res Inst Adv Mat & Green Chem Engn, Yantai 264006, Peoples R China;[4]Qingdao Ctr Resource Chem & New Mat, Qingdao 266100, Peoples R China

第一机构：Chinese Acad Sci, Lanzhou Inst Chem Phys, Res Ctr Nat Med & Chem Metrol, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Chinese Acad Sci, Lanzhou Inst Chem Phys, Res Ctr Nat Med & Chem Metrol, Lanzhou 730000, Peoples R China;[2]corresponding author), Yantai Zhongke Res Inst Adv Mat & Green Chem Engn, Yantai 264006, Peoples R China;[3]corresponding author), Qingdao Ctr Resource Chem & New Mat, Qingdao 266100, Peoples R China.

年份：2024

外文期刊名：ANALYTICAL METHODS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001299415700001)】；

基金：This work was supported by the Lanzhou planning project of science and technology (grant no. 2023-3-39), the China Agriculture Research System (grant no. CARS-9), the Central Government Guiding Local Science and Technology Development Project in 2023 (YDZX2023029), and the Gansu Planning Projects on Science and Technology (grant no. 23CXNJ0013).

语种：英文

摘要：As an important signaling molecule, carbon monoxide (CO) plays an important role in plant growth and development including affecting stomatal movement, stress response and root development. Thus, it is necessary to develop fluorescent probes that can be used to detect CO in live plant tissues and further enable a deep-understanding of its biological function, mechanism and metabolism. In this paper, a novel and sensitive fluorescent probe based on Cu2+ modulated polydihydroxyphenylalanine nanoparticles (PDOAs) has been developed for the detection of CO. The fluorescence of PDOAs can be effectively quenched by Cu2+ through the multi-coordination interaction. In the presence of CO, Cu2+ can be effectively reduced to Cu+, which resulted in the release of free PDOAs and the Cu2+-quenched bright green fluorescence was restored obviously. Through this ingenious strategy, the abiotic CO can be accurately detected and identified with high selectivity, rapid response time within 5 min and an ultralow detection limit of 72.4 nM. Due to the admirable biocompatibility, the nano-material based probe has been successfully applied for in vivo imaging CO in the root tip and leave tissues of lettuce. To the best of our knowledge, this is the first example of a fluorescent probe-based methodology for the sensitive tracking of CO in plant tissues.