文献类型：期刊文献

中文题名：红芪搓条前后主要次级代谢产物变化规律研究

英文题名：Variation rules of main secondary metabolites in Hedysari Radix before and after rubbing strip

作者：罗旭东[1];李昕蓉[1];李成义[1];齐鹏[1];梁婷婷[1];刘书斌[1];强正泽[1];何军刚[1];李旭[1];魏小成[1];冯晓莉[1,2];王明伟[1]

第一作者：罗旭东

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]敦煌医学与转化教育部重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2024

卷号：46

期号：3

起止页码：747

中文期刊名：中成药

外文期刊名：Chinese Traditional Patent Medicine

收录：CSTPCD;;北大核心:【北大核心2023】；CSCD:【CSCD2023_2024】；

基金：国家自然科学基金地区基金(81860683,82160730);国家自然科学基金青年科学基金(82104345);“双一流”科研重点项目(GSSYLXM-05);敦煌医学与转化教育部重点实验室项目(DHYX20-12);甘肃省重点研发计划项目(21YF5FA133)。

语种：中文

中文关键词：红芪;搓条;次级代谢产物;UPLC-MS/MS;聚类分析;主成分分析;正交偏最小二乘判别分析

外文关键词：Hedysari Radix;rubbing strip;secondary metabolites;UPLC-MS/MS;cluster analysis;principal component analysis;orthogonal partial least squares discriminant analysis

摘要：目的 考察红芪搓条前后主要次级代谢产物的变化规律。方法 UPLC-MS/MS法测定芒柄花素、芒柄花苷、毛蕊异黄酮、毛蕊异黄酮苷、美迪紫檀素、染料木素、木犀草素、甘草素、异甘草素、香草酸、阿魏酸、γ-氨基丁酸、腺苷、甜菜碱的含量,聚类分析、主成分分析、正交偏最小二乘判别分析进行化学模式识别以寻找差异性成分。结果 搓条后,芒柄花素、毛蕊异黄酮、甘草素、γ-氨基丁酸含量升高,芒柄花苷、毛蕊异黄酮苷、香草酸含量降低。搓条、未搓条药材聚为2类,毛蕊异黄酮苷、芒柄花素、γ-氨基丁酸、香草酸、毛蕊异黄酮、芒柄花苷为差异性成分。结论 本实验阐明红芪搓条前后化学成分差异,可为其他药材搓条机制研究提供参考。
AIM To investigate the variation rules of main secondary metabolites in Hedysari Radix before and after rubbing strip.METHODS UPLC-MS/MS was adopted in the content determination of formononetin,ononin,calycosin,calycosin-7-glucoside,medicarpin,genistein,luteolin,liquiritigenin,isoliquiritigenin,vanillic acid,ferulic acid,γ-aminobutyric acid,adenosine and betaine,after which cluster analysis,principal component analysis and orthogonal partial least squares discriminant analysis were used for chemical pattern recognition to explore differential components.RESULTS After rubbing strip,formononetin,calycosin,liquiritigenin andγ-aminobutynic acid demonstrated increased contents,along with decreased contents of ononin,calycosin-7-glucoside and vanillic acid.The samples with and without rubbing strip were clustered into two types,calycosin-7-glucoside,formononetin,γ-aminobutynic acid,vanillic acid,calycosin-7-glucoside and formononetin were differential components.CONCLUSION This experiment clarifies the differences of chemical constituents in Hedysari Radix before and after rubbing strip,which can provide a reference for the research on rubbing strip mechanism of other medicinal materials.