文献类型：期刊文献

中文题名：基于灰色关联分析的当归补血汤不同提取物体外抗氧化活性研究

英文题名：In vitro antioxidant activity of different extracts of Danggui Buxue Decoction(当归补血汤)based on grey relational analysis

作者：张宏美[1];王雅琼[1];赵彩霞[1];司姗姗[1];樊秦[1,2,3];张延英[1];张志瑞[1];康万荣[1];张璇[1];孙宇靖[1];戚欢阳[4];阎教科[5]

第一作者：张宏美

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]甘肃省中药质量与标准研究重点实验室,甘肃兰州730000;[3]敦煌医学与转化教育部重点实验室,甘肃兰州1730000;[4]厦门市健康医疗大数据中心,厦门市天然药物研究与开发重点实验室,福建厦门361008;[5]兰州美尔康生物科技有限公司,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2025

卷号：36

期号：12

起止页码：2244

中文期刊名：时珍国医国药

外文期刊名：Lishizhen Medicine and Materia Medica Research

收录：;北大核心:【北大核心2023】；

基金：兰州市人才创新创业项目(2022-RC-44);甘肃省中药质量与标准研究重点实验室开放基金项目(ZYZL2024-09);厦门市科技局一般科技计划项目(3502Z20224004)。

语种：中文

中文关键词：当归补血汤;抗氧化活性;指纹图谱;化学成分;熵权TOPSIS法;灰色关联分析

外文关键词：Danggui Buxue Decoction(当归补血汤);Antioxidant activity;Fingerprint;Chemical composition;Entropy weight TOPSIS method;Grey relational analysis

摘要：目的研究当归补血汤不同提取物HPLC指纹图谱及总多糖、总黄酮、总皂苷和总多酚与体外抗氧化活性之间的关系,明确发挥抗氧化活性的药效物质基础,筛选出最好的提取方法。方法建立当归补血汤不同提取物指纹图谱,测定4类化合物的含量;采用DPPH法、ABTS法和FRAP法测定不同提取物抗氧化活性;以含量和抗氧化药效为指标评价提取方法;利用灰色关联法分析当归补血汤不同提取物共有峰及4类化合物与综合抗氧化活性之间的相关性。结果建立了当归补血汤不同提取物HPLC指纹图谱,其中主要成分与4类化合物对抗氧化作用的大小顺序为总多酚>洋川芎内酯H>阿魏酸>洋川芎内酯I>总黄酮>总皂苷>芒柄花苷>芒柄花素>毛蕊异黄酮>未知成分1>总多糖>未知成分11>未知成分5>毛蕊异黄酮葡萄糖苷>绿原酸。结论基于灰色关联分析方法明确当归补血汤不同提取物抗氧化的药效是多种化学成分共同作用的结果,筛选出70%醇作溶剂回流提取方法最好。
Objective To investigate the HPLC fingerprints of different extracts of Danggui Buxue Decoction(当归补血汤,DGBXD)and the relationship between total polysaccharides,total flavonoids,total saponins and total polyphenols and antioxidant activity in vitro,to clarify the pharmacodynamic material basis for antioxidant activity,and to screen out the best extraction method.Methods Fingerprints of different extracts of DCBXD were established,and the contents of four types of compounds were determined.The antioxidant activities of different extracts were determined by DPPH,ABTS and FRAP.The content and antioxidant efficacy were used as indicators to evaluate the extraction method.The grey relational analysis(CRA)was used to analyze the correlation between the common peaks of different extracts of DGBXD,the four types of compounds and the comprehensive antioxidant activity.Results HPLC fingerprints of different extracts of DGBXD were established,and the order of antioxidant effect of the main components and four types of compounds was total polyphenols>senkyunolide H>ferulic acid>senkyunolide I>total flavonoids>total saponins>manganese>manganese ananthin>mullein isoflavones>unknown component 1>total polysaccharides>unknown component 11>unknown component 5>verbasin isoflavone glucoside>chlorogenic acid.Conclusion Based on GRA,it is clear that the antioxidant efficacy of different extracts of DGBXD is the result of the joint action of multiple chemical components,and the best method for solvent reflux extraction is to screen out 70%alcohol.