文献类型：期刊文献

中文题名：贞芪扶正颗粒联合奥沙利铂对H_(22)荷瘤小鼠的抑制效果及对MyD88、NF-κB p65的影响

英文题名：Tumor Inhibition Effect of Zhenqifuzheng Granule Combined with Oxaliplatin on H_(22)Tumor-bearing Mice and its Influence on MyD88 and NF-κB p65

作者：周熙祥[1];伍志伟[1,2];张录梅[1];刘永琦[1,2,3];薛娜[1];颜春鲁[1]

第一作者：周熙祥

机构：[1]甘肃中医药大学,兰州730000;[2]甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,兰州730000;[3]敦煌医学与转化省部共建教育部重点实验室,兰州730000

第一机构：甘肃中医药大学

年份：2020

卷号：36

期号：6

起止页码：166

中文期刊名：中药药理与临床

外文期刊名：Pharmacology and Clinics of Chinese Materia Medica

收录：北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：甘肃省科技创新基地与人才计划项目(编号:18JR3RA200);甘肃省高校重大疾病分子医学与中医药防治研究重点实验室开放基金项目(编号:FYZX17-18-14)。

语种：中文

中文关键词：贞芪扶正颗粒;奥沙利铂;H_(22)肝癌细胞;髓样分化因子;核因子-κB;肿瘤坏死因子

外文关键词：Zhenqifuzheng granule;Oxaliplatin;H_(22)hepatocellular carcinoma;MyD88;NF-κB p65;TNF-α

摘要：目的:观察贞芪扶正颗粒联合奥沙利铂对H_(22)移植瘤的抑制效果并探讨肿瘤相关髓样分化因子(Myeloid differentiation factor 88,MyD88)、核因子-κB(Nuclear Factor-κB p65,NF-κB p65)、肿瘤坏死因子(Tumor necrosis factor-α,TNF-α)的调节机制。方法:72只KM小鼠随机分为空白对照组、模型对照组、奥沙利铂10 mg/kg组、贞芪扶正颗粒0.026、0.013、0.007 g/kg+奥沙利铂10 mg/kg组,建立H_(22)荷瘤小鼠模型,经药物干预10 d后,剥取瘤体,进行瘤体测量、称重和抑瘤率分析;HE染色法观察瘤体组织形态学变化;Elisa法测定血清中TNF-α蛋白含量;WB和qPCR法分别检测瘤体中MyD88、NF-κB p65蛋白和mRNA水平。结果:与奥沙利铂10 mg/kg组相比,奥沙利铂10 mg/kg+贞芪扶正颗粒0.026、0.013、0.007 g/kg组瘤体体积和体质量均明显减小(P<0.05或P<0.01),抑瘤率上升;HE染色观察发现各治疗组坏死肿瘤细胞增多、细胞核碎裂并消失,其中0.026 g/kg组可见纤维组织增生;血清中TNF-α水平显著增高(P<0.01),瘤体组织中MyD88、NF-κB p65蛋白和mRNA水平显著下调(P<0.05),且呈现贞芪扶正颗粒浓度剂量依赖性关系。结论:贞芪扶正颗粒具有促进奥沙利铂抑制H_(22)移植瘤增长的作用,其分子机制可能与调节MyD88、NF-κB p65和TNF-α水平有关。
Objective:To observe the inhibitory effect of Zhenqifuzheng granule combined with Oxaliplatin on H_(22)-bearing mice and explore the regulatory mechanism of MyD88,NF-κB p65 and TNF-α.Methods:72 KM mice were randomly divided into:the cotrol group,the model group,10 mg/kg Oxaliplatin group,Zhenqifuzheng granule combined with 10 mg/kg Oxaliplatin groups(0.026,0.013,0.007 g/kg).H_(22)-bearing mice model was established,10 days after treatment,the tumors were dissected,measured and weighed to calculated the inhibitory rate.The histological changes of the tumor were observed by HE.TNF-αcontent in serum was measured by ELISA.The levels of MyD88,NF-κB p65 protein and mRNA in the tumor tissues were detected by WB and qPCR.Results:Compared with 10 mg/kg Oxaliplatin group,the volumes and weights of tumors were decreased in all doses of Zhenqifuzheng granule combined with oxaliplatin groups(0.026,0.013,0.007 g/kg)(P<0.05 or P<0.01)),while the inhibitory rate was increased.HE results showed that necrotic tumor cells were increased,the nucleus disintegrated and disappeared in all doses of combination groups,the fibrous tissue hyperplasia was observed in 0.026 g/kg combination group.The serum level of TNF-αwas significantly increased(P<0.01),the contents of MyD88,NF-κB p65 protein and mRNA in tumor tissues were significantly decreased(P<0.05),in concentration-dose-dependent manner.Conclusions:Zhenqifuzheng granule can promote Oxaliplatin to inhibit the growth of H_(22)cancer cell,its molecular mechanism may be related to regulating the levels.of MyD88,NF-κB p65 and TNF-α.