文献类型：期刊文献

中文题名：湿生扁蕾对溃疡性结肠炎肠纤维化大鼠结构及转化生长因子β表达的影响

英文题名：Effect of Gentianopsis paludosa on expressions of transforming growth factor-β and structure in ulcerative colitis fibrosis rats

作者：王雅莉[1];刘雪枫[2];张艳霞[3];景明[2];卢年华[2];刘娟娟[2]

第一作者：王雅莉

机构：[1]甘肃中医药大学基础医学院,兰州730000;[2]甘肃中医药大学药学院,兰州730000;[3]甘肃中医药大学甘肃省高校中(藏)药化学与质量研究省级重点实验室、甘肃中医药大学,兰州730000

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）

年份：2018

卷号：34

期号：8

起止页码：972

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金地区基金资助项目(81360522); 甘肃省自然科学基金资助项目(1606RJZA001)

语种：中文

中文关键词：湿生扁蕾;溃疡性结肠炎;纤维化;转化生长因子β

外文关键词：Gentianopsis paludosa; ulcerative colitis; fibrosis; transforming growth iactor - β

摘要：目的观察藏药湿生扁蕾对溃疡性结肠炎肠纤维化结构与转化生长因子β(TGF-β)表达的影响。方法按照体重将造模后大鼠随机分为5组,每组12只:模型组、阳性对照组和高、中、低3个剂量实验组;未进行模型制备的12只大鼠作为正常组。用2,4,6-三硝基苯磺酸造模。造模后24 h开始灌胃给药,正常组和模型组灌服等量0.9%Na Cl,阳性对照组灌服柳氮磺胺吡啶混悬液200mg·kg^(-1),3个剂量实验组灌服湿生扁蕾含生药5.6,2.8,1.4 g·kg^(-1),每天给药1次,连续2周。然后以苏木精伊红(HE)染色观察结肠组织结构改变,以Masson染色观察其纤维化程度,用免疫组织化学法观察TGF-β的表达。结果中剂量实验组与阳性对照组比较,湿生扁蕾能够减轻结肠纤维化的程度,使TGF-β表达减少。结论湿生扁蕾对溃疡性结肠炎肠纤维化有抑制作用,其作用机制可能与TGF-β表达减少有关。
Objective To investigate the effect of Gentianopsis paludosa on expressions of transforming growth factor-β（ TGF-β） and structure in ulcerative colitis（ UC） fibrosis rats. Methods The SD rats were randomly divided into five groups： normal,model,positive control and experimental-L,experimental-M and experimental-H groups,12 rats in each group. Fibrosis model rats was induced with trinitrobenzene sulphonic acid except for rats in normal group. Rats in experimental groups were administered with 50% ethanol solution and the corresponding drugs5. 6,2. 8,1. 4 g · kg-（-1）,respectively; rats in positive control group was administered sulfasalazine pyridine suspension 200 mg·kg-（-1）; rats in normal,model groups were administered equivalent 0. 9% Na Cl,by gavage for 2 weeks. At the end of the treatment,cell morphology was analyzed by hematoxylin eosin（ HE）. The fibrosis was analyzed by Masson. The expression of TGF-β was analyzed by immunohistochemistry. Results Comparison between positive control group and experimental-M group,the fibrosis of UC was obviously mitigated,TGF-β expression of UC fibrosis rats was significantly decreased. Conclusion The inhibitory effect on fibrosis was observed in experimental groups,which may be related to the expression of TGF-β.