文献类型：期刊文献

中文题名：藏药黑果枸杞水提物对运动疲劳所致氧化应激的影响

英文题名：Effects of Lycium Ruthenicum Murr. Aqueous Extract on Oxidative Stress Induced by Exercise Fatigue

作者：杨晓磊[1];耿嘉[1];仇道静[2];王昕[3];罗慧英[1,4]

第一作者：杨晓磊

机构：[1]甘肃中医药大学药学院,兰州730000;[2]甘肃省兰州市红古区海石湾窑街煤电公司医院海石分院,兰州730084;[3]兰州大学第二医院,兰州730030;[4]甘肃省中药药理与毒理重点实验室,兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2018

卷号：35

期号：10

起止页码：1494

中文期刊名：中国现代应用药学

外文期刊名：Chinese Journal of Modern Applied Pharmacy

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2017_2018】；

基金：甘肃省自然科学基金项目(1508RJZA005)

语种：中文

中文关键词：黑果枸杞;自由基;氧化应激;运动疲劳

外文关键词：Lycium ruthenicum Murr.;free radical;oxidative stress;exercise fatigue

摘要：目的研究藏药黑果枸杞水提物对运动疲劳所致氧化应激的影响。方法 150只小鼠随机分为3组,分别是泳前组、泳后即刻组和泳后恢复30 min组,每组50只。每组再分为5小组,每组10只,分别是阳性组、空白组和黑果枸杞高、中、低剂量组,灌胃给药30 d后测定小鼠泳前、泳后即刻和泳后恢复30 min后血清中丙二醛(malondialdehyde,MDA)、脂质过氧化物(lipidperoxide,LPO)、活性氧物质(reactiveoxygenspecies,ROS)含量及超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性。同时采用体外自由基发生体系,评价藏药黑果枸杞对1,1-二苯基-2-三硝基苯肼(DPPH)、羟基自由基(·OH)、超氧阴离子(O2-·)的清除力。结果黑果枸杞不但可以明显降低泳后及泳后恢复组小鼠血清中MDA、LPO、ROS含量,增加SOD、CAT、GSH-Px活性,而且可以减少DPPH、·OH、O2-·的产生。结论藏药黑果枸杞可明显缓解由于运动疲劳所引起的氧化应激反应。
OBJECTIVE To study the effects of Lycium ruthenicum Murr. aqueous extract on oxidative stress induced by exercise fatigue. METHODS One hundred and fifty mice were randomly divided into 3 groups, 50 in each group： no swimming group, immediate after swimming group and restore 30 min after swimming group. Mice in each group were subdivided into 5 groups of 10 each： positive control group, blank control group and Lycium ruthenicum Murr. low, medium and high dose group. After 30 d of intragastric administration, the contents of MDA, LPO and ROS, and the activities of SOD, CAT, GSH-Px in serum were measured. At the same time, the in vitro free radical generation system was used to evaluate the scavenging power of Lycium ruthenicum Murr. on 1,1-diphenyl-2-trinitrophenylhydrazine（DPPH）, ·OH and O2-·. RESULTS Lycium ruthenicum Murr. could not only reduce the contents of MDA, LPO and ROS in the serum of mice after swimming and 30 min after swimming significantly, but also increase the activities of SOD, CAT and GSH-Px. Moreover, it could reduce the production of DPPH, ·OH and O2-· radicals. CONCLUSION Lycium ruthenicum Murr. can alleviate the oxidative stress caused by exercise fatigue obviously.