文献类型：期刊文献

英文题名：Targeting thioredoxin reductase by eupalinilide B promotes apoptosis of colorectal cancer cells in vitro and in vivo

作者：Duan, Dongzhu[1];Guo, Xiangyu[1];Tian, Jingjing[1];Li, Mi[3,4];Jin, Xiaojie[3,4];Wang, Zihua[1,2];Wang, Le[1];Yana, Yunyun[1];Xiao, Jian[1];Song, Peng[5,6];Wang, Xiaoling[1]

第一作者：Duan, Dongzhu

通信作者：Wang, ZH[1];Xiao, J[1];Wang, XL[1];Song, P[2];Song, P[3]

机构：[1]Baoji Univ Arts & Sci, Coll Chem & Chem Engn, Shaanxi Key Lab Phytochemistry, Baoji 721013, Peoples R China;[2]Fujian Med Univ, Sch Basic Med Sci, Fujian Prov Key Lab Brain Aging & Neurodegenerat D, Fuzhou 350122, Peoples R China;[3]Gansu Univ Chinese Med, Sch Pharm, Lanzhou 730000, Peoples R China;[4]Gansu Univ Chinese Med, Gansu Univ Key Lab Mol Med & Chinese Med Prevent &, Lanzhou 730000, Peoples R China;[5]Gansu Univ Chinese Med, Key Lab Prevent & Treatment Chron Dis TCM Gansu P, Affiliated Hosp, Lanzhou 730000, Gansu, Peoples R China;[6]Key Lab Prevent & Treatment Chron Dis TCM, Lanzhou 730000, Gansu, Peoples R China

第一机构：Baoji Univ Arts & Sci, Coll Chem & Chem Engn, Shaanxi Key Lab Phytochemistry, Baoji 721013, Peoples R China

通信机构：[1]corresponding author), Baoji Univ Arts & Sci, Coll Chem & Chem Engn, Shaanxi Key Lab Phytochemistry, Baoji 721013, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Key Lab Prevent & Treatment Chron Dis TCM Gansu P, Affiliated Hosp, Lanzhou 730000, Gansu, Peoples R China;[3]corresponding author), Key Lab Prevent & Treatment Chron Dis TCM, Lanzhou 730000, Gansu, Peoples R China.|[10735b845793de6ae2b30]甘肃中医药大学第二附属医院;[10735]甘肃中医药大学;

年份：2024

卷号：399

外文期刊名：CHEMICO-BIOLOGICAL INTERACTIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001320302700001)】；

基金：This work was supported by the National Natural Science Foundation of China (82073727, 82073316, and 82260859), the Innovation Capability Support Program of Shaanxi (2022TD-63), the Innovation R&D & D Team of BUAS on Qinling Medicinal Plants and Functional Organic Molecules, the Education Department of Shaanxi Provincial Government (20JS007), Shaanxi Science and Technology Commission (2023-JC-YB-659), Gansu Province Science Foundation for Distinguished Young Scholars (20JR10RA348), and Natural Science Foundation of Gansu Province (22JR5RA611) .

语种：英文

外文关键词：Thioredoxin reductase; Eupalinilides B; Colorectal cancer; Oxidative stress; Apoptosis

摘要：Aberrant activation of thioredoxin reductase (TrxR) is correlated with tumor occurrence and progression, suggesting that TrxR inhibitors can be used as antitumor agents. In this study, we evaluated the anticancer efficacy of eupalinilides B on colorectal cancer cells. Eupalinilides B primarily targeted the conserved selenocysteine 498 residues in TrxR. Besides, it inhibited the enzyme activity in an irreversible manner. After eupalinilides B was used to pharmacologically inhibit TrxR, reactive oxygen species accumulated, and the intracellular redox balance was broken, finally causing oxidative stress-induced tumor cell apoptosis. Significantly, eupalinilides B treatment inhibited in vivo tumor growth. Targeting TrxR by eupalinilides B reveals the new mechanism underlying eupalinilides B and provides insight in developing eupalinilides B as the candidate antitumor chemotherapeutic agent for the treatment of cancer.