文献类型：期刊文献

中文题名：A549细胞外分泌物诱导间充质干细胞恶性分化的研究

英文题名：A Study on the Induction of Malignant Differentiation of Mesenchymal Stem Cells by Extracellular Secretions from A549 Cell

作者：杨玥[1];何建新[2,3];黄际宇[1];张利英[2,3];刘永琦[2,3]

第一作者：杨玥

机构：[1]西北民族大学医学部,甘肃兰州730030;[2]甘肃中医药大学基础医学院,甘肃兰州730000;[3]敦煌医学与转化教育部重点实验室,甘肃兰州730000

第一机构：西北民族大学医学部,甘肃兰州730030

年份：2025

卷号：23

期号：23

起止页码：1

中文期刊名：中国医药指南

外文期刊名：Guide of China Medicine

基金：国家自然科学基金青年基金(81503377);甘肃省教育厅产业支撑计划项目(2022CYZC-54);甘肃省高校青年博士基金(2022QB-027);西北民族大学引进人才科研项目(xbmuyjrc202235);甘肃中医药大学敦煌医学与转化教育部重点实验室开放基金(DHYX18-10)。

语种：中文

中文关键词：间充质干细胞;细胞增殖;细胞迁移;成瘤

外文关键词：Mesenchymal stem cell;Cell proliferation;Cell migration;Tumorigenecity

摘要：目的研究肺癌细胞A549条件培养液诱导间充质干细胞(MSC)在增殖、迁移、成瘤及侵袭方面的改变。方法设MSC为对照组,A549细胞条件培养液培养的MSC为模型组(T-MSC)。通过检测细胞形态、细胞增殖及迁移能力评估各组MSC发生恶性转化的程度;经Westernblot检测各组MSC恶性分化相关NF-κB及STAT3的活性;通过体内实验研究各组MSC的成瘤能力。结果T-MSC组在形态上出现改变,从纤维样漩涡状改变为椭圆形纺锤状;T-MSC组增殖能力和体外迁移能力高于对照组MSC(P<0.05);T-MSC组p65及STAT3蛋白磷酸化水平高于对照组MSC(P<0.05);T-MSC组细胞可在NOD-SCID小鼠皮下出现成瘤样表型且实体瘤表现侵袭浸润样表型。结论A549细胞条件培养液可诱导MSC发生一定程度的恶性分化,表现出细胞形态学改变、增殖及迁移能力提高、获得成瘤样表型,这可能是通过调控NF-κB及STAT3活性来实现的。
Objective Study the effects of conditional-medium from lung cancer cells A549 on proliferation,migration,tumorigenicity and invation of mesenchymal stem cells(MSC).Methods Grouping MSC as control group and A549 cells-conditioned medium-cultured MSC as model group(T-MSC).Evaluating the degree of malignant transformation in each group by detecting cell morphology,proliferation and migration.The malignant differentiation-associated NF-κB and STAT3 activities were detected by Western blot analysis in each group.The tumorigenicity of each group was detected in vivo.Results Cells in T-MSC group exert morphological changes fromfibrously swirls shape to elliptically spindle-like shape.Cells in T-MSC group exert higher proliferation and migration compared with cells in control group(P<0.05).Phosphorylation level of p65 and STAT3 in T-MSC group are higher than those in control group(P<0.05).Cells in T-MSC group exert tumorigenic phenotype and invasive and infiltration-like phenotype in NOD-SCID mice.Conclusions MSC cultured in A549 cells-conditioned medium exert malignant transformation in some degree,displaying morphological change,elevated proliferation and migration,tumor-like phenotype,which may be fulfilled though activating NF-κB and STAT3 in T-MSC.