文献类型：期刊文献

英文题名：Efficacy of Cigu Xiaozhi pill () on non-alcoholic steato-hepatitis-associated lipoapoptosis through stress-activated c-Jun N-terminal kinase signalling pathway

作者：Ma Yanhua[1];Han Tao[2];Yang Shaojun[3];Yu Chengzu[1];Shi Xia[4];Wu Yan[1]

第一作者：马燕花

通信作者：Ma, YH[1]

机构：[1]Gansu Univ Chinese Med, Coll Clin Med, Lanzhou 730000, Peoples R China;[2]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China;[3]Guangxi Beihai Hosp Tradit Chinese Med, Beihai 536000, Peoples R China;[4]Gansu Univ Chinese Med, Ctr Teaching Expt, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学临床医学院

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Coll Clin Med, Lanzhou 730000, Peoples R China.|[10735c1ddf597548aedb5]甘肃中医药大学临床医学院;[10735]甘肃中医药大学;

年份：2021

卷号：41

期号：1

外文期刊名：JOURNAL OF TRADITIONAL CHINESE MEDICINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000617101600010)】；

基金：Supported by the Regional Fund Project of National Natural Science Foundation of China (No. 81560753): the Effect of JNK/C-Jun Signaling Pathway on Fas Mediated Lipogenic Apoptosis of Stem Cells in Non-Alcoholic Steatohepatitis and the Intervention of Detoxification

语种：英文

外文关键词：Non-alcoholic fatty liver disease; JNK mitogen-activated protein kinases; apoptosis; stress-activated signaling pathway; Cigu Xiaozhi pill

摘要：OBJECTIVE: To investigate the efficacy of Cigu Xiaozhi pill (CGXZ) on non-alcoholic steatohepatitis (NASH)-associated lipoapoptosis through the stress-activated c-Jun N-terminal kinase (JNK)/ stress-activated protein kinase signalling pathway. METHODS: Sixty male Sprague-Dawley rats were randomly divided into the following groups (10 rats each): blank control, model, low-dose CGXZ, medium-dose CGXZ, high-dose CGXZ, and positive control (treated with SP600125, a JNK inhibitor). The NASH model was established and the histomor-phological characteristics of haematoxylin and eosin-stained liver tissues were examined under a light microscope. Cell apoptosis in liver tissues was assessed via terminal deoxynucleotidyl transferase dUTP nick-end labelling assay. In addition, the mRNA and protein expression levels of p-JNK, p-c-Jun, caspase-8, Fas, and Fas-L were determined via fluorescence-based quantitative real-time PCR, immunohistochemical and Western blot assays. RESULTS: Histopathological examination of the liver showed that the model rats had moderate-to-severe steatosis with infiltration of inflammatory cells as well as significantly higher expression levels of the p-JNK, p-c-Jun, caspase-8, Fas, and Fas-L proteins, compared with those in the blank control group (P < 0.01). Hepatic lobules of the rats in the treatment groups showed significantly reduced vacuolar degeneration and steatosis as well as alleviated inflammatory cell infiltration. The high and medium-dose CGXZ groups exhibited significantly lower mRNA and protein expression levels of p-JNK, p-c-Jun, caspase-8, Fas, and Fas-L, compared with those in the model group (P < 0.05 or P < 0.01). CONCLUSION: CGXZ pill inhibited the onset of hepatocyte apoptosis by regulating the expression of p-JNK, p-c-Jun, caspase-8, Fas, and Fas-L, thereby exerting therapeutic effects against NASH. (C) 2021 JTCM. All rights reserved.