文献类型：期刊文献

中文题名：基于代谢组学研究热补针法治疗类风湿关节炎寒证家兔的作用机制

英文题名：Mechanism of Heat-reinforcing Needling for Rheumatoid Arthritis Rabbits with Cold Syndrome Based on Metabonomics

作者：杜小正[1];袁博[1];张星华[2];田亮[1];张婷卓[1];李兴兰[1];张枫帆[1];王金海[3]

第一作者：杜小正

机构：[1]甘肃中医药大学针灸推拿学院,兰州730000;[2]甘肃省中医院针灸科,兰州730050;[3]兰州大学第二医院中医科,兰州730030

第一机构：甘肃中医药大学针灸推拿学院

年份：2017

卷号：42

期号：3

起止页码：202

中文期刊名：针刺研究

外文期刊名：Acupuncture Research

收录：CSTPCD;;Scopus(收录号：2-s2.0-85046007679);北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；PubMed(收录号：29071975);

基金：国家自然科学基金(No.81260558);国家中医药管理局甘肃郑氏针法学术流派传承工作室项目(No.2305135901)

语种：中文

中文关键词：热补针法;类风湿关节炎;代谢组学

外文关键词：Heat-reinforcing needling; Rheumatoid arthritis; Metabonomics

摘要：目的:采用代谢组学技术探讨热补针法治疗类风湿关节炎(RA)寒证的特异性作用机制。方法:青紫蓝家兔随机分为正常组、模型组、平补平泻组、捻转补法组、热补针法组,每组8只。以卵蛋白诱导联合低温冷冻法建立RA寒证模型。各治疗组分别运用平补平泻法、捻转补法、热补针法于双侧"足三里"穴进行针刺干预,治疗30min,每日1次,共治疗7d。治疗后观察各组家兔的痛阈值以及关节表面温度,采用气相色谱-质谱(GC-MS)检测结合模式识别法分析家兔血清中内源性代谢物的变化。结果:模型组家兔痛阈、关节表面温度较正常组降低(P<0.05),各针刺组干预后痛阈、关节表面温度较模型组升高(P<0.05),热补针法组高于平补平泻组、捻转补法组(P<0.05)。与正常组相比,模型组家兔血液代谢物中亮氨酸、缬氨酸、异亮氨酸、花生四烯酸的含量升高(P<0.05),α-酮戊二酸、柠檬酸、琥珀酸、葡萄糖、纤维糖、棕榈酸、硬脂酸、乳糖、d-核糖、D-甘露糖的含量降低(P<0.05)。与模型组比较,各针刺组血清中代谢物均出现不同程度的回调(P<0.05),且热补针法组对α-酮戊二酸、柠檬酸、琥珀酸、葡萄糖、纤维糖、d-核糖、D-甘露糖的回调明显优于平补平泻组和捻转补法组(P<0.05)。结论:热补针法治疗RA具有确切的疗效,可能与对三羧酸循环与糖代谢的相对特异性调控有关。
Objective To explore the specific mechanism of heat-reinforcing needling （HRN） for rheumatoid arthritis （RA） rabbits with cold syndrome based on metabolomics. Methods A total of 40 healthy rabbits were randomly divided into a normal group, a model group, a reinforcing-reducing needling（RRN） group, a twirling-reinforcing needling （TRN） group and a HRN group （ n = 8 rabbits/group）. The RA model with cold syndrome was established with ovalbumin and freezing. Except the normal and model groups, RRN, TRN and HRN were given at ＂Zusanli＂ （ST 36） in the corresponding acupuncture groups for 30 min, once a day for 7 days. After the interventions, the pain threshold and the local skin temperature of each group were observed. The en- dogenous metabolites in the serum were analyzed by the gas chromatography mass spectrometry （GO-MS） technology combined with the pattern-recognition method. Results The pain threshold and the local skin temperature in the model group were lower than those in the normal group （P〈0.05）. The pain thresholds and the local skin temperatures in the three acupuncture groups were higher than those in the model group after interventions （ P〈0.05）, and those in the HRN group were obviously higher than those in the RRN and TRN groups （P〈0.05）. The serum metabolites of leucine, valine, isoleucine, and arachidonic acid in the model group were increased compared with those in the normal group （P〈0.05）, and the metabolites of α-ketoglutaric acid, citric acid, succinic acid, glucose, inositol, palmitic acid, stearic acid, lactose, d-ribose, and D-mannose were decreased （P〈0.05）. The above-mentioned metabolites in the three acupuncture groups were significantly reversed relevant to the model group （P〈0.05）, and the effect of HRN group was significantly superior to those of the RRN and TRN groups in regulating α-ketoglutaric acid, citric acid, succinic acid, glucose, inositol, d-ribose , and D-mannose （P〈0.05）. Conclusion HRN for RA with cold syndrome is effective, which may be related to the specific regulation for the krebs cycle and glycometabolism.