文献类型：期刊文献

英文题名：Nalbuphine Pretreatment Improves Myocardial Cell Viability Induced by H/R Through TLR4/NLRP3-Mediated Pyroptosis

作者：Chang, Yanna[1];Xie, Hai[2];Gao, Zhijie[3];Liu, Huimin[3]

第一作者：畅艳娜

通信作者：Xie, H[1]

机构：[1]Gansu Univ Chinese Med, Dept Anesthesia & Surg, Affiliated Hosp, Lanzhou 730000, Gansu, Peoples R China;[2]Lanzhou Univ, Hosp 1, Dept Emergency, 1 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China;[3]Lanzhou Univ, Clin Med Coll 1, Lanzhou 730099, Gansu, Peoples R China

第一机构：甘肃中医药大学第二附属医院

通信机构：[1]corresponding author), Lanzhou Univ, Hosp 1, Dept Emergency, 1 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China.

年份：2022

卷号：46

期号：3

起止页码：739

外文期刊名：IRANIAN JOURNAL OF SCIENCE AND TECHNOLOGY TRANSACTION A-SCIENCE

收录：;Scopus(收录号：2-s2.0-85128837795);WOS:【SCI-EXPANDED(收录号:WOS:000787627200001)】；

基金：This work was supported by Affiliated Hospital of Gansu University of Chinese Medicine and The First Hospital of Lanzhou University. There is no funding support.

语种：英文

外文关键词：Myocardial ischemic-reperfusion injury; Nalbuphine; Cell viability; Pyroptosis; TLR4; NLRP3

摘要：Myocardial ischemic-reperfusion injury (MIRI) has been the nonnegligible obstacle for the treatment of myocardial infarction that is the dominating cause of morbidity and mortality in cardiovascular diseases around the world. To explore the potential therapeutic drugs and the underlying molecular mechanisms of the MIRI, hypoxia/reoxygenation (H/R) was used to treat H9c2 cardiomyocyte cells to simulate MIRI. The cell viability of H9c2 cardiomyocyte cells was a dose-dependent reduction with nalbuphine, and the maximum non-toxic concentration of nalbuphine was 200 mu M. H/R treatment significantly decreased cell viability and increased the rate of pyroptosis and pyroptosis-related proteins levels of H9c2 cardiomyocyte cells, which was prominently reversed with 200 mu M nalbuphine pretreatment. Moreover, TLR4 overexpression prominently reduced the cell viability and enhanced the rate of pyroptosis and pyroptosis-related proteins levels of H9c2 cardiomyocyte cells based on the pretreatment of nalbuphine. Therefore, nalbuphine pretreatment improved myocardial cell viability induced by H/R through TLR4/NLRP3-mediated pyroptosis.