文献类型：期刊文献

中文题名：中国沙棘雄花花被及花粉总黄酮提取工艺优化及抗氧化活性研究

英文题名：Optimization of total flavonoids extraction process and study on antioxidant activities from the perianth and pollen of Hippophae rhamnoides L.subsp.sinensis Rousi male flower

作者：牟佳勤[1];杨文玺[1,2,3];叶亮[1];成春亚[1];刘彩霞[1];李妙[1];崔秋圆[1];张启立[1,2,3];崔治家[1,2,3];邵晶[1,2,3]

第一作者：牟佳勤

机构：[1]甘肃中医药大学药学院,兰州730000;[2]甘肃省中医药研究中心,兰州730000;[3]西北中藏药省部共建协同创新中心,兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2026

卷号：17

期号：6

起止页码：216

中文期刊名：食品安全质量检测学报

外文期刊名：Journal of Food Safety and Quality

基金：甘肃省自然科学基金项目(22JRRA1724);甘肃省中医药研究中心开放课题项目(zyzx-2023-17);甘肃省现代农业产业技术体系建设专项(GSARS06)。

语种：中文

中文关键词：中国沙棘雄花;花粉;花被;总黄酮;提取工艺;抗氧化活性

外文关键词：Hippophae rhamnoides L.subsp.sinensis Rousi male flower;pollen;perianth;total flavonoids;extraction process;antioxidant activity

摘要：目的优化中国沙棘雄花花被及花粉总黄酮的提取工艺,并比较其抗氧化活性。方法采用超声辅助醇提法提取花被和花粉的总黄酮,利用分光光度法测定总黄酮含量,结合单因素实验和响应面优化二者最佳提取工艺,同时开展体外实验,对中国沙棘雄花花被和花粉的总黄酮开展1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基和2,2’-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)[2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)radical cation,ABTS]阳离子自由基清除能力进行研究。结果花被的最佳提取工艺为60%的乙醇体积分数,液料比10:1(mL:g),61℃超声提取39 min,此条件下花被总黄酮提取率为(4.59±0.12)%;花粉最佳提取工艺为乙醇浓度70%,液料比10:1(mL:g),52℃超声提取62 min,此条件下花粉的总黄酮提取率为(1.88±0.06)%。在质量浓度为4.0 mg/mL时,花被、花粉总黄酮DPPH自由基清除率分别为(84.87±0.03)%、(82.53±0.11)%,在质量浓度为0.80 mg/mL时,花被、花粉ABTS阳离子自由基清除率分别为(83.21±0.13)%、(79.37±0.06)%。结论本研究分析了中国沙棘雄花花被和花粉在总黄酮含量和抗氧化能力方面的差异,结果表明花被的总黄酮含量和抗氧化能力均高于花粉,且二者均表现出较好的抗氧化能力,二者的差异性为花被花粉分别开发利用提供了思路,同一性为中国沙棘雄花整体资源的开发利用提供了数据支持。
Objective To establish the optimal extraction process of total flavonoids from the perianth and pollen of Hippophae rhamnoides L.subsp.sinensis Rousi male flowers and compare their antioxidant activity.Methods The total flavonoids of perianth and pollen were extracted by ultrasonic-assisted alcohol extraction method.The content of total flavonoids was determined by spectrophotometry.The optimal extraction process of both was optimized by single factor experiment and response surface optimization.Additionally,in vitro experiments were conducted to evaluate the 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical and the 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)cationic radical scavenging activities of total flavonoids from the calyx and pollen.Results The optimal extraction process for the perianth was 60%ethanol by volume,a solid-to-liquid ratio of 10:1(mL:g),ultrasonic extraction at 61°C for 39 min,under which the total flavonoid yield was(4.59±0.12)%.The optimal extraction process for pollen was 70%ethanol,solid-to-liquid ratio of 10:1(mL:g),ultrasonic extraction at 52°C for 62 min,under which the total flavonoid yield was(1.88±0.06)%.At a mass concentration of 4.0 mg/mL,the DPPH radical scavenging rates of total flavonoids from perianth and pollen were(84.87±0.03)%and(82.53±0.11)%,respectively.At a mass concentration of 0.80 mg/mL,the ABTS cationic radical scavenging rates of total flavonoids from perianth and pollen were(83.21±0.13)%and(79.37±0.06)%,respectively.Conclusions This study analyzes the differences in total flavonoid content and antioxidant capacity between the perianth and pollen of male flowers from Hippophae rhamnoides L.subsp.sinensis Rousi.The results showes that the total flavonoid content and antioxidant capacity of the perianth were higher than those of the pollen,and both exhibited good antioxidant capacity.The differences provide ideas for the separate development and utilization of the perianth and pollen,while the similarities offer data support for the overall development and utilization of male flowers from Hippophae rhamnoides L.subsp.sinensis Rousi resources.