文献类型：期刊文献

中文题名：甘南藏族人群G蛋白耦联受体激酶4基因多态性与血压盐敏感性的关系

英文题名：Association of G protein-coupled receptor kinase 4 gene polymorphism with salt sensitivity of blood pressure in a Gannan Tibetan population

作者：曹静[1];陈凭[2];郑贵森[1];王云[1];刘希波[1];靳利梅[1];邢新[1];胡继宏[1,3]

第一作者：曹静

机构：[1]甘肃中医药大学公共卫生学院,甘肃兰州730000;[2]甘肃省兰州市第二人民医院体检中心;[3]甘肃中医药大学科研实验中心

第一机构：甘肃中医药大学公共卫生学院

年份：2020

卷号：28

期号：3

起止页码：257

中文期刊名：中华高血压杂志

外文期刊名：Chinese Journal of Hypertension

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：国家自然科学基金项目(81260444)。

语种：中文

中文关键词：藏族人群;血压盐敏感性;G蛋白耦联受体激酶4基因

外文关键词：Tibetan;salt sensitivity of blood pressure;G protein-coupled receptor kinase 4 gene

摘要：目的研究甘南藏族自治州藏族人群的G蛋白耦联受体激酶4(GRK4)基因多态性与血压盐敏感性的关系。方法 2013年6月到2014年11月期间,采用多级抽样的方法,在甘肃省甘南藏族自治州夏河县和合作县中随机抽取符合原发性高血压诊断标准的787名藏民作为病例组,同时随机抽取正常血压藏民660人作为对照组,对其进行问卷调查、体格检查和盐敏感性检测。采用我国改良后的急性盐负荷试验方法进行盐敏感性判定;采集外周静脉血6 mL,提取DNA,检测GRK4基因单核苷酸多态性(SNP)。根据血压和盐敏感性情况,将研究对象分为4组:盐敏感性高血压组(n=554)、盐不敏感性高血压组(n=233)、盐敏感性正常血压组(n=423)与盐不敏感性正常血压组(n=237)。比较4组人群一般情况及各基因位点上不同基因型和等位基因的分布情况,采用Logistic回归分析GRK4基因与血压盐敏感性之间的关系。结果 4组人群在GRK4基因位点rs12506119和rs1419043上的基因型以及等位基因的分布差异有统计学意义(均P<0.05)。以盐不敏感性正常血压组作为对照,在校正混杂因素[年龄、性别、文化程度、职业、吸烟、饮酒、体育锻炼以及体质量指数(BMI)]后,多因素Logistic回归分析结果显示,与rs1419043位点的GG基因型相比,C等位基因突变(CG+CC基因型)是盐敏感性高血压的保护因素(OR=0.06, 95%CI 0.01~0.93,P=0.04)。结论甘南藏族人群GRK4基因的rs1419043位点与盐敏感性高血压有关。
Objective To investigate the association of G protein-coupled receptor kinase 4(GRK4) gene polymorphism with salt sensitivity of blood pressure in a Tibetan population of Gannan Tibetan Autonomous Prefecture. Methods From June 2013 to November 2014, a total of 787 Tibetans with essential hypertension and 660 Tibetans with normal blood pressure were enrolled using multistage random sampling method. The information was collected by questionnaires, physical examination and salt sensitivity test. The salt sensitivity was determined using the improved acute salt load test method in China. The DNA was extracted from 6 mL of peripheral venous blood and tested for the single nucleotide polymorphisms(SNP) of GRK4. According to blood pressure and salt sensitivity, the subjects were divided into four groups including salt-sensitive hypertension(n=554), non-salt-sensitive hypertension(n=233), salt-sensitive normotension(n=423) and non-salt-sensitive normotension(n=237). The differences in characteristics and distribution of genotypes and alleles at each locus among four groups were analyzed. Logistic regression was used to analyze the relationship between GRK4 gene and the salt sensitivity of blood pressure in Tibetan population. Results The distribution of genotypes and allele frequencies in rs12506119 and rs1419043 were significantly different among four groups(all P<0.05). Non-salt-sensitive normotension was used as control, after adjusting for confounding factors [age, gender, education, occupation, smoking, drinking, physical exercise and body mass index(BMI)], multivariate Logistic regression analysis showed that C allele mutation(CG+CC genotype) was a protective factor for salt-sensitive hypertension group compared with GG genotype at rs1419043(OR=0.06, 95% CI=0.01-0.93, P=0.04). Conclusion GRK4 gene locus rs1419043 may be associated with the occurrence of salt-sensitive hypertension in the Tibetan population of Gannan.