文献类型：期刊文献

中文题名：蜜煎导通便栓对大鼠润肠通便的作用及其机制

英文题名：Effects of Mijiandao suppository on intestinal laxation in rats and its mechanisms

作者：杜晓凤[1];陈升富[2];商婷[1];倪志涛[1];史玉攀[1];王谨慧[1]

第一作者：杜晓凤

机构：[1]甘肃中医药大学附属医院,兰州730000;[2]甘肃中医药大学药学院,兰州730000

第一机构：甘肃中医药大学第二附属医院

年份：2022

卷号：38

期号：6

起止页码：776

中文期刊名：中国应用生理学杂志

外文期刊名：Chinese Journal of Applied Physiology

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；PubMed;

基金：甘肃省自然科学基金资助项目(20JR10RA340);全国中药特色技术传承人才培训项目(T20184828005)。

语种：中文

中文关键词：蜜煎导通便栓;润肠通便;复方地芬诺酯;水通道蛋白;5-羟色胺

外文关键词：Mijiandao suppository(MJDs);Moisturizing intestines;compound diphenoxylate;aquaporins;5-hydroxytryptamine(5-HT)

摘要：目的:探索蜜煎导通便栓(MJDs)对复方地芬诺酯诱发雄性大鼠便秘模型的润肠通便作用及机制。方法:将60只SD雄性大鼠,随机分为空白对照组、模型组、阳性组、MJDs组。采用复方地芬诺酯灌胃法复制便秘模型,空白对照组及模型组灌肠生理盐水,阳性组灌肠给予开塞露,MJDs组灌肠给予蜜煎导通便栓,连续灌肠给药10 d,每天一次。造模及给药期间对大鼠体质量、粪便含水量、胃排空率、碳墨推进率等指标进行观察;采用苏木素-伊红(HE)染色观察MJDs对便秘大鼠结肠组织病理变化的影响;采用ELISA试剂盒观察MJDs对便秘大鼠结肠中5-羟色胺(5-HT)的影响;采用免疫组化法检测MJDs对便秘大鼠结肠中水通道蛋白(AQPs)3、水通道蛋白(AQPs)4表达的影响。结果:给药10 d后,与空白对照组比较,模型组体重、粪便含水量、碳墨推进率、结肠5-HT含量显著降低,结肠AQP3、AQP4表达量显著增高(P<0.05,P<0.01);与模型组比较,阳性组粪便含水量与结肠5-HT含量显著性增加,结肠AQP3、AQP4表达量显著下降,MJDs组体重、粪便含水量与结肠5-HT含量显著升高,AQP3、AQP4表达量显著下降(P<0.05,P<0.01);与阳性组比较,MJDs组粪便含水量显著下降,MJDs组结肠AQP3、AQP4表达量显著性下降(P<0.05,P<0.01);胃排空率各组间差异无统计学意义。结论:MJDs对便秘具有良好的通便作用,其机制可能与上调结肠5-HT含量,下调结肠AQP3及AQP4表达有关。
Objective:To investigate the effects of Mijian Daotong Bowel Suppository(MJDs)on the compound diphenoxylate induced constipation model of male rats and its mechanisms.Methods:Sixty SD male rats were randomly divided into blank group,model group,positive group and MJDs group.The constipation model was established by using compound diphenoxylate gavage.The rats in blank group and model group were treated with saline by enema,the rats in positive group and MJDs group were given Kaisailu and honey decoction laxative suppository by enema,respectively,once a day for 10 days.The body weight,fecal water content,gastric emptying rate(GER)and carbon ink propulsion rate(CIPR)of rats were observed during modeling and administration.The effects of MJDs on the pathological changes of colon tissue in constipation rats were investigated by hematoxylin-eosin(HE)staining.The effect of MJDs on 5-hydroxytryptamine(5-HT)in the colon of constipation rats was investigated by ELISA kit.The effects of MJDs on the expressions of aquaporins 3(AQP3)and aquaporins 4(AQP4)in the colon of constipation rats were detected by immunohistochemistry.Results:After 10 days of administration,compared with the blank group,the body weight,fecal water content,carbon ink propulsion rate and colon 5-HT content in the model group were decreased significantly,while the expression levels of AQP3 and AQP4 in the colon were increased significantly(P<0.05,P<0.01).Compared with the model group,the fecal water content and colon 5-HT content in the positive group were increased significantly,and the expressions of AQP3 and AQP4 in the colon were decreased significantly.The body weight,fecal water content and colon 5-HT content in the MJDs group were increased significantly,and the expressions of AQP3 and AQP4 was decreased significantly(P<0.05,P<0.01).Compared with the positive group,the fecal water content of the MJDs group was decreased significantly,and the expressions of AQP3 and AQP4 in the colon of the MJDs group was decreased significantly(P<0.05,P<0.01).Gastric emptying rate was not statistically significant difference between the groups.Conclusion:MJDs has good therapeutic effects on constipation,and its mechanisms may be related to up-regulating the content of 5-HT in the colon and down-regulating the expressions of AQP3 and AQP4 in the colon.