文献类型：期刊文献

中文题名：当归挥发油对自发性高血压大鼠心肌组织中微小RNA差异表达谱的影响

英文题名：Effect of Angelica Sinensis Volatile Oil on miRNA differential expression profiling in myocardium tissue in spontaneously hypertensive rats

作者：伊琳[1];陈蓓蓓[1];孙少伯[2];杨陇[3];纪禄风[1];石向慧[1]

第一作者：伊琳

机构：[1]甘肃中医药大学中西医结合学院内科学教研室,甘肃兰州730000;[2]甘肃中医药大学基础医学院医用生物学教研室,甘肃兰州730000;[3]甘肃省通渭县人民医院外科,甘肃通渭743300

第一机构：甘肃中医药大学临床医学院|甘肃中医药大学中西医结合学院

年份：2016

卷号：42

期号：4

起止页码：671

中文期刊名：吉林大学学报（医学版）

外文期刊名：Journal of Jilin University：Medicine Edition

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：国家自然科学基金资助课题(81460669);甘肃省科技厅第十批科技计划项目资助课题(1310RJZA084)

语种：中文

中文关键词：当归挥发油;大鼠,近交SHR;微小RNA表达谱

外文关键词：Angelica Sinensis Volatile Oil; rat; inbred SHR; miRNA expression profiling

摘要：目的：探讨当归挥发油对自发性高血压大鼠（SHRs）心肌组织中微小RNA（miRNA）表达谱的影响,阐明当归挥发油发挥作用的可能机制。方法：将SHRs分为模型组、卡托普利组和当归组,取同周龄的正常Wistar大鼠作为对照组,采用无创套尾法测定大鼠尾动脉收缩压,给药4周后,采用Affymetrix miRNA 4.0芯片测定心肌组织中miRNA表达谱,采用京东基因与基因组百科全书（KEGG）富集法分析差异表达的miRNA靶基因。结果：给药前模型组大鼠收缩压明显高于对照组（P〈0.05）,连续服药1～4周后,卡托普利组和当归组大鼠收缩压明显低于模型组（P〈0.05）。与模型组比较,当归组大鼠差异表达的miRNA 29个,其中表达上调的miRNA 13个（Ratio〉2.0,P〈0.05）,表达下调的miRNA 16个（Ratio〈0.5,P〈0.05）。KEGG富集分析,miR-19a、let-7i和miR-181c与胰岛素信号通路有关联（P〈0.05）,let-7i、miR-181a和miR-455与血管内皮生长因子（VEGF）信号通路调控有关联（P〈0.05）,miR-122、miR-181a、miR-200b、miR-181c、let-7i和miR-19a与细胞凋亡有关联（P〈0.05）。结论：当归挥发油可降低SHRs血压,对SHRs心肌组织中miRNA表达谱产生影响,可能通过调节胰岛素信号通路及VEGF信号通路相关miRNA发挥血压调节作用。
Objective：To explore the effect of Angelica Sinensis Volatile Oil on the mi RNA expression profiling in myocardium tissue of the spontaneously hypertensive rats（SHRs）,and to clarify the possible mechanism of Angelica Sinensis Volatile Oil.Methods：All the SHRs were divided into Angelica group,model group and captopril group,and other Wistar rats with the same age were selected as control group.The non-invasive systolic blood pressure was detected;after 4weeks of administration,the changes of miRNA expression profiling in myocardium tissue were measured by Affymetrix miRNA 4.0 Array.KEGG analysis was used to identify the target genes.Results：Compared with control group,the systolic blood pressure of the rats in model group was significantly decreased（P〈0.05）before treatment.4 weeks after administration,compared with model group,the systolic blood pressure of the rats in Angelica group was significantly decreased（P〈0.05）.Compared with model group,29 differential miRNAs of rats in Angelica group were found（P〈0.05）,with 13up-regulated miRNAs and16down-regulated miRNAs.The KEGG analysis results showed that miR-19 a,let-7i,and miR-181 cwere related to insulin signaling pathways;let-7i, miR-181 a,and miR-455 were related to VEGF signaling pathways;miR-122,miR-181 a, miR-200 b, miR-181 c,let-7i,and miR-19 a were related to apoptosis（P〈0.05）.Conclusion：Angelica Sinensis Volatile Oil can decrease the blood pressure in SHRs and it can regulate the blood pressure by regulating the miRNA related to insulin signaling pathways and VEGF signaling pathways.