文献类型：期刊文献

英文题名：Overexpressing Arabidopsis KNAT1 gene in Celosia plumosus L. causes modification of plant morphology

作者：Sun, Shao-Bo[1];Song, Jiang-Ping[2];Yang, Jing[3]

第一作者：孙少伯

通信作者：Sun, SB[1]

机构：[1]GanSu Coll Tradit Chinese Med, Lanzhou 730000, Peoples R China;[2]ShaoXing Univ, Sch YuanPei, Shaoxing 312000, Peoples R China;[3]Lanzhou Univ, Hosp 2, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), GanSu Coll Tradit Chinese Med, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2011

卷号：33

期号：5

起止页码：1597

外文期刊名：ACTA PHYSIOLOGIAE PLANTARUM

收录：;Scopus(收录号：2-s2.0-80051672380);WOS:【SCI-EXPANDED(收录号:WOS:000293894600005)】；

基金：This research was specially supported by GanSu College of Traditional Chinese Medicine and ShaoXing University.

语种：英文

外文关键词：KNAT1; Celosia plumosus L.; KNAT1-GFP protein; Arabidopsis; Dwarfism; Lobed or rumpled leaves

摘要：Celosia plumosus L. is one of the most common gardening plants worldwide. The C. plumosus L. transformation system established by other research team should be useful for modifying physiological, medicinal, and horticultural traits. Though morphological modification of C. plumosus L. would be critical in terms of its commercial value, few attempts have been reported until now. In modification of gardening plants, many potentially useful genes that are involved in the pathways associated with flower and plant morphology have been cloned. Transcription factors regulating plant development and biosynthetic or regulatory genes involved in plant hormones are common candidates. KNAT1, isolated from Arabidopsis, is a knotted1-like homeobox (knox), and it is expressed in the shoot apical meristem and not in determinate organs. Previous reports have indicated that ectopic expression of KNAT1 in Arabidopsis changes simple leaves into lobed leaves or rumpled leaves. Therefore, this transcription factor might regulate plant morphology. In this work, we constructed the binary vector pBIN-pMD-18T, which contained the GFP and KNAT1 coding sequence, and transformed them into C. plumosus L. plants by Agrobacterium tumefaciens. Our results show that the KNAT1-GFP fusion protein was selectively located in the nucleus of roots and leaves, consistent with a potential function as transcription factor reported by other research teams. We observed that some 35S:KNAT1-GFP plants display lobed or rumpled leaves, partite leaves. Moreover, overall plant morphology was altered in extreme phenotype category, i.e., dwarfed. Together, these morphological modifications of C. plumosus L. can have potential practical applications.