文献类型：期刊文献

英文题名：Neuroinflammatory mechanism of Volatile oil from Acori graminei Rhizoma in tourette syndrome: GC-MS, network pharmacology and experimental validation

作者：Sun, Kexin[1];Wei, Xing[1];Feng, Peng[2];Cui, Min[1];Huang, Shuang[1];Jiang, Bing[1];Su, Qian[1];Ding, Yanqin[3];Tian, Yongyan[4];Tan, Jiaxin[5];He, Yuezhen[6];Shang, Jing[1];Shi, Zhenggang[1]

第一作者：Sun, Kexin

通信作者：Shi, ZG[1];Feng, P[2]

机构：[1]Gansu Univ Chinese Med, Clin Coll Chinese Med, Lanzhou 730000, Gansu, Peoples R China;[2]Hexi Univ, Med Coll, Zhangye 734000, Gansu, Peoples R China;[3]Hexi Univ, Zhangye Peoples Hosp, Paediat, Zhangye 734000, Gansu, Peoples R China;[4]Hexi Univ, Silk Rd Tradit Chinese Med Res Ctr, Zhangye 734000, Gansu, Peoples R China;[5]Ningxia Med Univ, Gen Hosp, Dept Emergency Med, Yinchuan 750000, Ningxia, Peoples R China;[6]Lanzhou Univ, Hosp 1, Dept Tradit Chinese Med, Lanzhou 730000, Gansu, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Clin Coll Chinese Med, Lanzhou 730000, Gansu, Peoples R China;[2]corresponding author), Hexi Univ, Med Coll, Zhangye 734000, Gansu, Peoples R China.|[10735]甘肃中医药大学;

年份：2025

卷号：265

外文期刊名：JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

收录：;Scopus(收录号：2-s2.0-105011389375);WOS:【SCI-EXPANDED(收录号:WOS:001543109300001)】；

基金：This work was supported by National Natural Science Foundation Regional Project (grant number 82460953) ; Gansu University of Chinese Medicine Doctoral Supervisor Open Fund Project (grant number ZYXKBD-202204) ; Gansu Provincial Science and Technology Plan Funding Project (grant number 22JR11RG221) ; Hexi University Doctoral Research Start-up Fund Project (grant number KYQD2022015) ; Gansu Provincial Administration of Traditional Chinese Medicine Research Project (grant number GZKP-2023-55) ; Hexi University Pres-ident's Fund Youth Research Project (grant number QN2023001) ; Gansu Provincial Higher Education Youth Doctoral Fund Project (grant number 2023QB-075) ; Gansu Provincial College Teachers' Innovation Fund Project (grant number 2024-B-098) .

语种：英文

外文关键词：Volatile oil from Acori graminei Rhizoma; Tourette syndrome; Neuroinflammation; Microglia; CAMP/PKA/CREB signaling pathway

摘要：Tourette syndrome (TS) is a neurodevelopmental disorder characterized by tics that significantly impair childhood development. Volatile oil from Acori graminei Rhizoma (VOA), known for its anti-inflammatory and neuroprotective properties, holds therapeutic potential for TS, though its molecular mechanisms remain undefined. In this study, VOA was extracted via hydrodistillation (yield: 1.03 %), and the TS rat model was established to evaluate behavioral improvements through intragastric administration of varying VOA doses. The high-dose group (most effective in alleviating tics) was selected for subsequent mechanistic investigations. Utilizing GC-MS data from our group, network pharmacology and molecular docking analyses prioritized the cAMP/PKA/ CREB signaling pathway as a potential target. Behavioral assessments confirmed that VOA significantly alleviated stereotyped and motor behaviors. In the striatum, VOA reduced pro-inflammatory cytokines, attenuated neuronal damage, and shifted microglial polarization from M1 to M2 phenotypes. From a mechanistic perspective, VOA upregulates key components in the cAMP/PKA/CREB signaling pathway and enhances BDNF levels, while suppressing the expression of NF-kappa B p65 and phosphorylation levels. These findings demonstrate that VOA mitigates tic behaviors by activating the cAMP/PKA/CREB signaling pathway, thereby curbing neuroinflammation, rectifying MG polarization, and preserving neuronal integrity. This study elucidates the critical link between TS pathogenesis and neuroinflammatory dysregulation, unveiling VOA's molecular interplay with the cAMP/PKA/CREB signaling pathway and offering novel therapeutic insights for TS.