文献类型：期刊文献

中文题名：灵芝多糖对NB4细胞凋亡及Egr-1表达的影响

英文题名：Effect of Ganoderma lucidum Polysacchrides on Apoptosis and Egr-1 Expression in NB4 Cells

作者：李广远[1];杨雅丽[1];李海龙[1];楚惠媛[1];李雪燕[1];陈彻[1];王晶[1]

第一作者：李广远

机构：[1]甘肃中医学院

第一机构：甘肃中医药大学

年份：2013

卷号：24

期号：5

起止页码：476

中文期刊名：中药新药与临床药理

外文期刊名：Traditional Chinese Drug Research and Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD_E2013_2014】；

基金：甘肃省教育厅基金项目(044B-05)

语种：中文

中文关键词：灵芝多糖;急性白血病;凋亡;Egr-1

外文关键词：Ganoderma lucidum polysacchrides; Acute promyelocytic leukemia; Apoptosis; Egr-1

摘要：目的研究灵芝多糖在体外对人类白血病细胞株NB4细胞的增长抑制及凋亡的影响及抗肿瘤的机制。方法用MTT比色法检测灵芝多糖对NB4细胞增长的抑制作用,Annexin V-FITC/PI双染色法检测NB4细胞的凋亡情况,荧光定量PCR及蛋白印迹技术检测早期生长反应因子1(Egr-1)基因的表达。结果灵芝多糖对NB4细胞增殖具有抑制作用并可促其凋亡,最高分别为33.5%及18.77%。这种效应呈时间-剂量依赖性。与对照组比较,差异有统计学意义(P<0.01)。对Egr-1 mRNA和蛋白表达也具有明显的促进作用,差异有统计学意义(P<0.01)。结论灵芝多糖在体外能够明抑制NB4细胞的增长并促其凋亡,其机制可能与上调Egr-1的表达相关。
Objective To investigate the effects of Ganoderma lucidum polysacchrides（GLP） on the proliferation and apoptosis of human acute promyelocytic leukemia NB4 cells in vitro, and to explore the possible anti-tumor mechanism. Methods The inhibitory effects of GLP on the proliferation of NB4 cells were measured by using MTT assay. The labeled Annexin V-FITC/PI and flow cytometry were used to detect the apoptotic cells. The expression levels of Egr-1 mRNA and protein were evaluated by fluorescent quantitation PCR and Western blotting, respectively. Results GLP inhibited the proliferation of NB4 cells and promoted its apoptosis in vitro, and the rate being 33.5 %, 18.77 % respectively. The effect was time- and dose-dependent （P 〈 0.01 compared with the control group）. The expression levels of Egr-1 mRNA and protein were aslo significantly up-regulated（P 〈 0.01 ）. Conclusion GLP can inhibit the proliferation of NB4 cells and promoted its apoptosis in vitro, and the mechanism could be related to up-regulation of Egr-l expression.