文献类型：期刊文献

中文题名：阿魏酸对人胃癌MGC-803细胞增殖的影响

英文题名：Effects of Ferulic Acid on Gastric Cancer Cell Line MGC-803 Proliferation

作者：张艳[1];李海龙[2];王虎平[1,2];顾静[2];马春林[1,2];吴红彦[1,2]

第一作者：张艳

机构：[1]甘肃中医药大学基础医学院;[2]甘肃省中医方药挖掘与创新转化重点实验室甘肃省中药新产品创制工程实验室

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）

年份：2016

卷号：23

期号：9

起止页码：70

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2015_2016】；

基金：甘肃省自然科学基金(1212RJZA083)

语种：中文

中文关键词：阿魏酸;胃癌;细胞增殖;细胞凋亡

外文关键词：ferulic acid;gastric cancer;cell proliferation;cell apoptosis

摘要：目的观察阿魏酸对人胃癌MGC-803细胞增殖的影响,探讨其诱导胃癌细胞凋亡的作用机制。方法不同浓度阿魏酸干预体外培养人胃癌MGC-803细胞,作用24、48、72 h后,MTT法检测细胞增殖;不同浓度(0、5、7.5、10 mg/m L)阿魏酸作用MGC-803细胞48 h,Annexin V-FITC/PI法和流式细胞仪检测细胞凋亡,RT-q PCR和Western-blot检测Capase-3、Capase-9、Bax、Bcl-2和Xiap的m RNA和蛋白表达。结果与对照组比较,5、7.5、10、12.5 mg/m L阿魏酸作用MGC-803细胞的OD值明显降低,阿魏酸抑瘤作用明显,阿魏酸作用MGC-803细胞24、48、72 h的IC50分别为12.93、9.73、5.52 mg/m L;5、7.5、10 mg/m L阿魏酸作用MGC-803细胞48 h后均能诱导MGC-803细胞凋亡,5、7.5、10 mg/m L阿魏酸均能上调Capase-3、Capase-9和Bax的m RNA和蛋白表达,下调Bcl-2和Xiap的m RNA和蛋白表达。结论阿魏酸能有效抑制MGC-803细胞增殖,并能诱导MGC-803细胞凋亡,其作用机制与内源性线粒体凋亡途径和下调Xiap的表达有关。
Objective To explore the effects of ferulic acid on gastric cancer cell line MGC-803 proliferation; To discuss the mechanism of apoptosis induced by ferulic acid. Methods Ferulic acid with a variety of concentrations was used to treat gastric cancer cell line MGC-803 for 24, 48, 72 h; MTT experiment was used to detect the growth of gastric cancer cells. After gastric cancer cell line MGC-803 were treated with ferulic acid with a variety of concentrations（0, 5, 7.5, 10 mg/m L） for 48 h, all gastric cancer cells were collected and stained by Annexin V-FITC/PI for the detection of apoptosis by flow cytometry. RT-q PCR and Western-blot methods were used to detect m RNA and protein levels of Caspase-3, Caspase-9, Bax, Bcl-2 and Xiap. Results Compared with the control group, the OD value of cell line MGC-803 treated by ferulic acid with a variety of concentrations（5, 7.5, 10, 12.5 mg/m L） decreased significantly; the anti-tumor effect of ferulic acid was obvious; IC50 of 24, 48, and 72 hours after treated by ferulic acid was 12.93, 9.73 and 5.52 mg/m L respectively. Cell line MGC-803 treated by ferulic acid with a variety of concentrations（5, 7.5, 10 mg/m L） after 48 h could induce the apoptosis of MGC-803 cells, up-regulate m RNA and protein levels of Caspase-3, Caspase-9, and Bax, down-regulate m RNA and protein levels of Bcl-2 and Xiap. Conclusion Ferulic acid can inhibit the proliferation of MGC-803 cells effectively and induce the apoptosis of MGC-803 cells, which mechanism is related to mitochondria apoptosis pathway and the down-regulation of Xiap expression.