文献类型：期刊文献

中文题名：当归挥发油对小鼠的降压作用及血管活性的观察

英文题名：Antihypertensive and vasoactive effects of Angelica volatile oil on mice

作者：吴国泰[1,2];杜丽东[1,2];高云娟[1];景琪[1];庞丽[1];李应东[1];任远[1,2]

第一作者：吴国泰

机构：[1]甘肃中医学院;[2]甘肃省中药药理与毒理学重点实验室

第一机构：甘肃中医药大学

年份：2014

卷号：34

期号：13

起止页码：1045

中文期刊名：中国医院药学杂志

外文期刊名：Chinese Journal of Hospital Pharmacy

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金："十二五"国家科技支撑计划项目子课题(编号:2011BAI05B02)

语种：中文

中文关键词：当归挥发油;高血压小鼠;L-NNA;冷激;钙通道

外文关键词：Angelica volatile oil; hypertensive mice;L-NNA; cold shock; calcium channel

摘要：目的：观察当归挥发油对小鼠的降压作用和血管活性。方法：采用腹腔注射（ip）或静脉注射（iv）当归挥发油乳剂（含当归挥发油0.5%、0.25%,v/v）,连续观察2h内正常小鼠血压和心率的变化;采用灌胃左旋N-硝基精氨酸（L-NNA）700mgkg-1d-1和冷激（4h/d）分别建立2种高血压小鼠模型,灌胃当归挥发油乳剂（含当归挥发油0.5 %、0.25 %、0.125 %,v/v）10d,观察各组小鼠血压和心率的变化,研究当归挥发油对小鼠的降压作用;采用大鼠离体主动脉螺旋条,梯度给予当归挥发油乳剂（含当归挥发油1.0×10-8～1.0×10-3mlml-1）,研究当归挥发油对去氧肾上腺素（Phe）或KCl引发的血管收缩活性的影响。结果：iv当归挥发油乳剂后,在0～120min内,小鼠SBP、MBP、DBP水平均呈现降低的趋势,但是ip当归挥发油乳剂后,则呈现上升的趋势,在120 min时,iv大、小剂量组小鼠SBP、MBP、DBP明显降低、ip大、小剂量组小鼠SBP、MBP、DBP明显升高（P〈0.05）;与L-NNA模型组、冷应激模型组比较,当归挥发油高、中剂量组SBP、MBP、DBP水平明显降低（P〈0.05,P〈0.01）;当归挥发油对Phe诱发血管平滑肌收缩具有抑制作用,IC50分别为 3.12×10-5 mlml-1,、IC50为 1.84×10-5 mlml-1,当归挥发油对KCl诱导的血管平滑肌收缩具有抑制作用,IC50分别为3.60×10-5mlml-1、2.69×10-5mlml-1。结论：当归挥发油对正常小鼠的血压具有不确定的影响,但对高血压模型小鼠具有一定的降压作用,对ROCC和VOCC介导的主动脉平滑肌收缩均有抑制作用,呈现非特异性钙通道阻滞作用。
OBJECCTIVE To observe the antihypertensive and vasoactive effects of Angelica volatile oil emulsion on mice. METHODS 160 mice were randomly divided into 4 groups and were intraperitoneally injected （ip） or intravenously injected （iv） with Angelica volatile oil emulsion（containing Angelica volatile oil 0. 5 %, 0. 25 %, ml. ml-1）, blood pressure and heart rate were continuously observed for 2 h in normal mice. Using L-N- nitro arginine （L-NNA）700 mg. kg-1. d-l or cold stimulation（4 h.d-1）, 2 kinds of hypertensive model mice were established, and intragastric administration with Angelica volatile oil emulsion （containing Angelica volatile oil emulsion 0. 5 %, 0. 25 %, 0. 125%, ml. ml-1） for 10 d, blood pressure and heart rate were observed in mice. Isolated rat aorta strips were used, and Angelica volatile oil emulsion given gradiently（containing An- gelica volatile oil 1.0 ×10-8 ～ 1.0 × 10-3 ml· ml-1）. The effects of Angelica volatile oil emulsion on phenylephrine（Phe）or KC1 inducing vasoconstrictor activity were observed. RESULTS After giving Angelica volatile oil emulsion 0 - 120min, SBP, MBP, DBP levels showed a decreasing trend in the iv group, but showed a rising trend in the ip group, At 120 min, the SBP, MBP were significantly decreased in the iv group, SBP, MBP, DBP increased significantly in the ip group （P〈0. 05） ; SBP, MBP, DBP decreased significantly in the L-NNA model group or cold stress model group given with Angelica volatile oil of high, middle dose（P〈0. 05, P〈0. 01） ; Phe induced vascular smooth muscle contraction, IC50 of the Angelica volatile oil were 3.12× 10-5 ml·ml-1, and 1.84 × 10-5 ml· ml-1, KC1 induced vascular smooth muscle contraction, IC50 of the Angelica volatile oil were 3.60 × 10-5 ml-ml-1, 2. 69 ×10-5 ml. ml-1. CONCLUSION The antihypertensive effect of the Angelica volatile oil in normal mice is uncertain, but its antihypertensive effect on hypertension model mice is certain, and the inhibitory effects on both ROCC and VOCC mediated aortic smooth muscle contraction are certain also.