文献类型：期刊文献

英文题名：Ultrasound-assisted deep eutectic solvent extraction of kukoamine A and kukoamine B from Lycii cortex

作者：Wang, Xiaomei[1];Hu, Jinxia[2,3];Ma, Ling[4];Han, Zhiming[4];Liu, Jianfei[2,3];Lu, Zhibin[2,3];Pei, Dong[2,3];Wang, Ningli[2,3];Di, Duolong[1,2,3]

第一作者：王霄梅

通信作者：Di, DL[1];Pei, D[2];Wang, NL[2];Di, DL[2]

机构：[1]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China;[2]Chinese Acad Sci, Lanzhou Inst Chem Phys, CAS Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China;[3]Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China;[4]Ningxia Inst Drug Control, Yinchuan 750001, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China;[2]corresponding author), Chinese Acad Sci, Lanzhou Inst Chem Phys, CAS Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2025

卷号：66

起止页码：136

外文期刊名：PHYTOCHEMISTRY LETTERS

收录：;Scopus(收录号：2-s2.0-86000176924);WOS:【SCI-EXPANDED(收录号:WOS:001443693800001)】；

基金：This research was supported by Key R & D Program of Ningxia Hui Autonomous Region (2023BEG03060) , Gansu Science and Technology Major Project (22ZD6FA021) , Yunnan Province Major Scientific and Technological Project (202302AE090007 & 202402AA310034) , Gansu Provincial Pharmaceutical Research Project (2022GSMPA0043) and Key R & D Program of Yunnan Province (202203AD150003) .

语种：英文

外文关键词：Deep eutectic solvent; Lycii cortex; Kukoamine A; Kukoamine B; Ultrasonic-assisted extraction; Extraction mechanism

摘要：Deep eutectic solvents (DES), are new environmentally friendly and efficient extraction solvents, and have considerable importance for environmental protection and sustainable development. In this study, ultrasound- assisted extraction (UAE) using DES was carried out to extract kukoamine A (KuA) and kukoamine B (KuB) from Lycii cortex (LyC). The DES was synthesised using a magnetic stirring method. A novel approach to extract KuA and KuB from LyC was developed by combining UAE with DES. Different DES formulations were tested to evaluate the efficiency of KuA and KuB extraction. Among them, choline chloride-lactic acid (ChCl-LA) DES had the best effect, and its extraction rate was much higher than that of methanol, which was 8.5 times that of methanol. Both single factorial experiments and response surface methodology (RSM) were used to optimise and model the extraction process. Density functional theory was also used to discuss the extraction mechanism. It was found that HBD and HBA will interact with hydrogen bonding and van der Waals interactions during the formation of DES, and these interactions will also affect the decrease in electron density. Taking ChCl-LA and ChClurea as examples, it was found that the binding energy of ChCl-LA was lower than that of ChCl-urea, indicating that ChCl-LA had a stronger interaction with the target substance and a higher extraction rate. The combination of UAE-DES proved to be a sustainable, effective, and simple extraction technique for the recovery of KuA and KuB from LyC.