文献类型：期刊文献

中文题名：红芪多糖对脾虚型DGP大鼠肠道黏膜屏障的保护作用

英文题名：Protective effect of hedysarum polybotrys polysacchcaide on the intestinal mucosal barrier of rats with spleen deficiency DGP

作者：魏昭晖[1];万生芳[1];李荣科[1];郭倩[1];马欣欣[1]

第一作者：魏昭晖

机构：[1]甘肃中医药大学基础医学院,甘肃兰州730000

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）

年份：2025

卷号：41

期号：4

起止页码：522

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金地区基金资助项目(82060914);甘肃省优秀博士生基金资助项目(23JRRA1225);甘肃中医药大学研究生创新基金资助项目(2022-07)。

语种：中文

中文关键词：红芪多糖;脾虚;糖尿病胃轻瘫;肠黏膜屏障

外文关键词：hedysarum polybotrys polysacchcaide;spleen deficiency;diabetic gastroparesis;intestinal mucosal barrier

摘要：目的探讨红芪多糖(HPS)对脾虚型糖尿病胃轻瘫(DGP)大鼠肠黏膜屏障的保护作用。方法用多因素联合小剂量腹腔多次注射链脲佐菌素(STZ)法制备脾虚型DGP大鼠模型。将大鼠随机分为空白组(纯水灌胃)、模型组(纯水灌胃)、阳性对照组(0.09 g·kg^(-1)盐酸二甲双胍缓释片)和高、中、低剂量实验组(0.20、0.10、0.05 g·kg^(-1)HPS),各组均每天灌胃给药1次,连续8周。检测血糖,用酶联免疫吸附实验(ELISA)法检测血清中二胺氧化酶(DAO)、脂多糖(LPS)、D-乳酸(D-LA)含量,用逆转录聚合酶链反应(RT-PCR)和蛋白质印迹法检测回肠组织中闭锁蛋白1(Claudin-1)、闭合蛋白(Occludin)、连接复合物蛋白1(ZO-1)mRNA及蛋白相对表达水平。结果空白组、模型组、阳性对照组和高剂量实验组的血糖分别为(5.04±0.40)、(30.71±1.21)、(18.63±6.72)和(19.90±3.30)mmol·L^(-1),血清DAO分别为(49.56±6.13)、(192.19±24.40)、(130.63±19.90)和(120.24±17.53)pg·mL^(-1),LPS分别为(41.11±4.56)、(99.67±6.63)、(64.51±8.59)和(63.07±4.89)ng·L^(-1),D-LA分别为(506.45±52.22)、(1826.49±224.17)、(1166.47±121.78)和(1344.82±130.65)μg·L^(-1),Claudin^(-1)mRNA相对表达水平分别为1.03±0.32、0.25±0.12、0.94±0.40和0.71±0.21,Occludin mRNA相对表达水平分别为1.07±0.48、0.26±0.06、1.23±0.42、0.99±0.47,ZO-1 mRNA相对表达水平为1.00±0.13、0.43±0.18、0.85±0.07和0.69±0.08,Claudin-1蛋白相对表达水平分别为1.00±0.00、0.21±0.19、0.56±0.31和0.87±0.31,Occludin蛋白相对表达水平分别为1.01±0.27、0.38±0.11、0.88±0.10和0.85±0.18,ZO-1蛋白相对表达水平分别为1.00±0.14、0.43±0.04、0.77±0.02和0.67±0.16。模型组的上述指标与空白组比较,在统计学上差异均有统计学意义(P<0.01,P<0.05);高剂量实验组的上述指标与模型组比较,在统计学上差异均有统计学意义(P<0.01,P<0.05)。结论HPS可降低脾虚型DGP大鼠肠黏膜损伤,维持脾虚型DGP大鼠肠道黏膜屏障完整性。
Objective To investigate the protective effect of hedysarum polybotrys polysacchcaide(HPS)on intestinal mucosal barrier in rats with splenic deficiency type diabetic gastroparesis.Methods The rat model of spleen deficiency DGP was prepared by multifactorial combined with low-dose intraperitoneal injection of Stretocin.The rats were randomly divided into blank group(pure water gavage),model group(pure water gavage),positive control group(0.09 g·kg^(-1)metformin hydrochloride sustained-release tablet)and experimental-H,-M,-L groups(0.20,0.10,0.05 g·kg^(-1)HPS),each group was administered by gavage once a day for 8 W.Measured blood glucose,diamine oxidase(DAO),lipopolysaccharides(LPS),D-lactate contents(D-LA)in serum by enzyme linked immunosorbent assay method;Claudin-1,Occludin,Zonula Occluden-1(ZO-1)mRNA and protein expressions in lleal tissue were detected by reverse transcription-polymerase chain reaction and Western blot.Results The blood glucose in the blank group,model group,positive control group,experimental-H,-M,-L groups were(5.04±0.40),(30.71±1.21),(18.63±6.72)and(19.90±3.30)mmol·L^(-1);the DAO were(49.56±6.13),(192.19±24.40),(130.63±19.90)and(120.24±17.53)pg·m L^(-1);the LPS were(41.11±4.56),(99.67±6.63),(64.51±8.59)and(63.07±4.89)ng·L^(-1);the D-LA were(506.45±52.22),(1826.49±224.17),(1166.47±121.78)and(1344.82±130.65)μg·L^(-1);the relative expression levels of Claudin-1 mRNA were1.03±0.32,0.25±0.12,0.94±0.40 and 0.71±0.21;the relative expression levels of Occludin mRNA were1.07±0.48,0.26±0.06,1.23±0.42 and 0.99±0.47;the relative expression levels of ZO-1 mRNA were1.00±0.13,0.43±0.18,0.85±0.07 and 0.69±0.08;the relative expression levels of Claudin-1 protein were1.00±0.00,0.21±0.19,0.56±0.31 and 0.87±0.31;the relative expression levels of Occludin protein were1.01±0.27,0.38±0.11,0.88±0.10 and 0.85±0.18;the relative expression levels of ZO-1 protein were1.00±0.14,0.43±0.04,0.77±0.02 and 0.67±0.16.Compared with the the blank group,the above indexes in the model group had statistical significance(P<0.01,P<0.05);compared with the model group,the above indexes in the experimental-H had statistical significance(P<0.01,P<0.05).Conclusion HPS can reduce intestinal mucosal injury and maintain the integrity of the intestinal mucosal barrier in spleen deficiency DGP rats.