文献类型：期刊文献

英文题名：Transcriptomic elucidation of Dahuang-Huanglian in promoting white adipose browning in high-fat diet-induced obese rats

作者：Zhang, Ruiyao[1];Zhang, Yu[1];Xi, Xi[1];Du, Pengcheng[1];Guo, Chao[1,2];Zhang, Yanying[1,2];Song, Bing[1,2];Xu, Xiaoyan[1,3];Ni, Zhitao[1,3];Wang, Yongfeng[1,4];Bai, Min[5]

第一作者：张仁义

通信作者：Wang, YF[1];Wang, YF[2];Bai, M[3]

机构：[1]Gansu Univ Chinese Med, Lanzhou, Peoples R China;[2]Gansu Prov Tech Ctr Lab Anim, Lanzhou, Peoples R China;[3]Gansu Univ Chinese Med, Affiliated Hosp, Lanzhou, Peoples R China;[4]Gansu Med Coll, Pingliang, Peoples R China;[5]Ningxia Med Univ, Yinchuan, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Lanzhou, Peoples R China;[2]corresponding author), Gansu Med Coll, Pingliang, Peoples R China;[3]corresponding author), Ningxia Med Univ, Yinchuan, Peoples R China.|[10735]甘肃中医药大学;

年份：2025

卷号：16

外文期刊名：FRONTIERS IN ENDOCRINOLOGY

收录：;Scopus(收录号：2-s2.0-105019329806);WOS:【SCI-EXPANDED(收录号:WOS:001596909500001)】；

基金：The author(s) declare financial support was received for the research and/or publication of this article. This study was supported by Natural Science Foundation of Gansu Province (22JR5RA581, 23JRRA1227), Industrial Support Program of Gansu Provincial Education Department (2024CYZC-42), Open Fund Project of Gansu Provincial Key Laboratory of Traditional Chinese Medicine Formula Exploration and Innovation Transformation (FYWJ-24-08, FYWJ-24-10), Gansu Provincial Traditional Chinese Medicine Research Project (GZKP-2023-19), Scientific Research Startup Foundation for Introduced Talents of Gansu University of Chinese Medicine (2024YJRC-13), Gansu Provincial Postgraduate "Innovation Star" Program (2025CXZX-944), Gansu University of Chinese Medicine Postgraduate "Innovation and Entrepreneurship Fund" Project (2025CXCY-068).

语种：英文

外文关键词：transcriptomics; Dahuang-Huanglian; AMPK; white adipose tissue browning; obesity

摘要：Objective Dahuang (Rhei Radix et Rhizoma)-Huanglian (Coptidis Rhizoma) (DHHL), has been shown to effectively treat obesity caused by dietary irregularities. Nevertheless, the fundamental process driving this phenomenon has yet to be elucidated.Methods The chemical constituents of DHHL were analyzed using UPLC-MS/MS. An obesity model was established in rats by high-fat diet (HFD) induction and verified accordingly. Obese rats were administered various doses of DHHL. Detect and record the metabolic indicators of rats in each group. Transcriptomic analysis was used to evaluate the influence of DHHL on gene expression in obese rats. H&E staining and transmission electron microscopy (TEM) was used to observe the morphology of adipocytes. Immunohistochemistry (IHC), fluorescent immunohistochemistry (FIHC), and Western blotting (WB) were performed to detect protein expression levels.Results The chemical constituents of DHHL medicinal materials were identified and analyzed using UPLC-MS/MS. Total ion chromatograms (TIC) were acquired in both positive and negative ion modes. Pie charts were generated to illustrate the abundance distribution and quantitative proportion of different components. HFD feeding induced significant increases in body weight and FBG in rats, elevated serum triglycerides (TG) and free fatty acids (FFA) levels, and promoted hypertrophy and hyperplasia of adipose tissue, while also disrupting glucose metabolism. DHHL treatment significantly improved body weight, FBG, glucose uptake capacity, and insulin sensitivity in obese rats. It also reduced blood lipid levels and lipid accumulation in a dose-dependent manner. Transcriptomic sequencing revealed that the anti-obesity effects of DHHL were closely associated with the upregulation of thermogenesis-related gene expression. KEGG pathway enrichment analysis indicated that DHHL may exert regulatory effects through pathways such as AMPK, PPAR, and PI3K. TEM observations demonstrated that DHHL increased mitochondrial numbers within adipocytes of obese rats. Molecular analyses further showed that DHHL upregulated the expression of thermogenesis-associated proteins-including PPAR gamma, PRDM16, and UCP1-thereby promoting the browning of white adipose tissue (WAT). Moreover, DHHL enhanced the expression levels of AMPK, SIRT1, and PGC-1 alpha.Conclusions DHHL effectively ameliorates HFD-induced obesity in rats, and its therapeutic mechanism is closely associated with the activation of the AMPK/SIRT1/PGC-1 alpha signaling pathway, which promotes the browning of WAT.