文献类型：期刊文献

中文题名：蛇葡萄素钠协同卡铂体外诱导人肺腺癌GLC-82细胞凋亡研究

英文题名：Experimental study on apoptosis induced by ampelopsin sodium combined with carboplatin on human lung adenocarcinoma cell line GLC-82

作者：张艳霞[1,2];吴勇杰[2];李文广[2]

第一作者：张艳霞

机构：[1]甘肃中医学院;[2]兰州大学基础医学院药理学研究所甘肃省新药临床前研究重点实验室

第一机构：甘肃中医药大学

年份：2014

卷号：30

期号：4

起止页码：26

中文期刊名：中药药理与临床

外文期刊名：Pharmacology and Clinics of Chinese Materia Medica

收录：北大核心:【北大核心2011】；CSCD:【CSCD_E2013_2014】；

语种：中文

中文关键词：蛇葡萄素钠;卡铂;人肺腺癌GLC-82细胞;细胞凋亡;Bcl-2;Bax

外文关键词：ampelopsin sodium （蛇葡萄素钠） ; carboplatin; human lung adenocarcionma cell line GLC-82 ; apoptosis; Bcl-2 ; Bax

摘要：目的:考察蛇葡萄素钠单用及与卡铂合用对人肺腺癌GLC-82细胞增值的抑制作用,初步探讨蛇葡萄素钠抑瘤作用机制。方法:应用MTT法考察蛇葡萄素钠单用及与卡铂合用对人肺腺癌GLC-82细胞的细胞毒作用;使用流式细胞仪检测凋亡相关基因Bax以及Bcl-2表达。结果:MTT实验结果表明,蛇葡萄素钠单独应用及与卡铂联合应用对GLC-82细胞的增殖均具有浓度依赖性的抑制作用;流式细胞仪检测结果表明,蛇葡萄素钠(100、50、25μg/ml)单用组及与卡铂25μg/ml联合用药组作用于GLC-82细胞48 h后,Bax表达上调,且有浓度依赖性;AMP-Na 50μg/ml单独用药组、卡铂25μg/ml单独用药组以及AMP-Na(50、25、12.5μg/ml)与卡铂25μg/ml联合用药组作用于GLC-82细胞48 h后,Bcl-2蛋白的表达下调,但无浓度依赖性。AMP-Na各剂量组(100、50、25、12.5μg/ml)单用组及AMP-Na各剂量组与卡铂25μg/ml联合用药Bax/Bcl-2的比率增高,且有浓度依赖性。结论:蛇葡萄素钠单用对GLC-82细胞的增殖具有一定的抑制作用,与卡铂合用,对GLC-82细胞增殖具有协同抑制效应;蛇葡萄素钠可能是通过下调Bcl-2并上调Bax从而促进肿瘤细胞凋亡。
To investigate the cytotoxic effect and mechanism of ampelopsin sodium on human lung adenocarcionma cell line GLC-82 by alone or combined with carboplatin. Methods： Use the MTF colorimetric method to investigate cytotoxicity effect of ampelopsin sodium alone or combined with carboplatin to human lung adenocarcionma cell llne GLC-82 ; Use flow cytomctry（FCM） analysis the express of Bax and Bcl- 2. Results： Experimental study on cytotoxicity in vitro showed that, ampelopsin sodium（ 100, 50, 25 μg/ml） alone or combined with carbo- platin 25 μg/ml all had concentration dependent depressant effect to the generation of GLC-82 cell; FCM results showed that, 48 hours after ampelopsin sodium（ 100, 50, 25, 12.5 μg/ml）combined with carboplatin25 μg/ml to GLC-82 cell, the express of Bax concentration de- pendent up-regulation; the express of Bcl-2 in ampelopsin sodium（50 μ/ml） group, carboplatin 25μ/ml group, ampelopsin sodium（50, 25, 12.5 μg/ml ） combined with carboplatin 25 μg/ml group down regulation, but there, s no concentration dependent; the proportion of Bax/Bcl-2 raise up in all the group. Conclusion： Ampelopsin sodium had depressant effect to the generation of GLC-82 cell, There is a syn- ergistic cytotoxic effect on GLC-82 cells treated with ampelopsin sodium combined with carboplatin. The mechanism of ampelopsin sodium is probably that it can down regulation the express of Bcl-2 and up-regulation express of Bax.