文献类型：期刊文献

中文题名：短发夹RNA沉默丝裂原活化蛋白激酶1对胃癌细胞生物学表型的影响及其分子机制

英文题名：Effect of sh RNA silencing mitogen-activated protein kinase 1 on biological phenotype of gastric cancer cells and relevant biological mechanism

作者：王转兄[1];宋耀辉[1];杜正平[1];陈熙[1];顾静[2];李海龙[1]

第一作者：王转兄

机构：[1]甘肃中医药大学临床医学院临床检验诊断学教研室,甘肃兰州730000;[2]甘肃中医药大学基础医学院生理教研室,甘肃兰州730000

第一机构：甘肃中医药大学临床医学院

年份：2021

卷号：34

期号：9

起止页码：1047

中文期刊名：中国生物制品学杂志

外文期刊名：Chinese Journal of Biologicals

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD_E2021_2022】；

基金：甘肃省自然科学基金项目(20JR5RA189,17JR5RA173);甘肃省高等学校科研项目(甘财教[2014]63-15)。

语种：中文

中文关键词：短发夹RNA;胃癌;丝裂原活化蛋白激酶1;生物表型

外文关键词：shRNA;Gastric cancer;Mitogen-activated protein kinase(MAPK)1;Biological phenotype

摘要：目的探讨短发夹RNA(short hairpin RNA,shRNA)沉默丝裂原活化蛋白激酶1(mitogen-activated protein kinase 1,MAPK1)对胃癌细胞生物学表型的影响及其分子机制。方法 SGC-7901和HGC-27细胞按MOI=20分别感染慢病毒LV-sh-MAPK1、LV-sh-Ctrl(分别为两种细胞的试验组及阴性对照组)。RT-qPCR及Western blot法检测SGC-7901及HGC-27细胞中MAPK1的沉默效果,MTT法检测细胞的增殖能力,细胞划痕愈合试验和Transwell小室试验检测细胞的迁移能力,Transwell小室试验检测细胞的侵袭能力,流式细胞术检测细胞周期及凋亡,Western blot检测细胞周期及凋亡相关信号因子的表达情况。结果与阴性对照组比较,试验组细胞中MAPK1基因m RNA及蛋白表达水平均显著降低(P<0.01);细胞增殖、侵袭、迁移能力均显著降低(P<0.01);凋亡率显著增加(P<0.01),且细胞阻滞于G1期;CDK6、Bax、Caspase 3、C-Caspase 3、Caspase 9、C-Caspase 9的表达水平明显上调(P<0.01),CDK1、CCNB1、Bcl-2表达水平明显下调(P<0.01)。结论沉默MAPK1可抑制人胃癌细胞的增殖、侵袭、迁移能力,促进细胞凋亡,并将其阻滞在G1期。
Objective To investigate the effect of shRNA silencing mitogen-activated protein kinase(MAPK) 1 on biological phenotype of human gastric cancer cells as well as the relevant molecular mechanism.Methods SGC-7901 and HGC-27 cells were infected with lentivirus LV-sh-MAPK1 at a MOI of 20,using lentivirus LV-sh-Ctrol as negative control.The silencing efficiency of MAPK1 expression was determined by RT-qPCR and Western blot,while the cell proliferation by MTT assay,the cell migration by cell healing test,the cell invasion ability by Transwell assay,the cell cycle and apoptosis by flow cytometry,and the expressions of cycle-and apoptosis-related signaling factors by Western blot.Results Compared with those in negative control group,both mRNA transcription and protein expression levels of MAPK1 as well as the cell proliferation,invasion and migration abilities in test group decreased significantly(P<0.01),while the apoptosis rate increased significantly(P<0.01),and the cell cycle were arrested at G1 phase;the expression levels of CDK6,Bax,Caspase 3,C-Caspase3,Caspase 9 and C-Caspase9 was significantly up-regulated(P<0.01),while those of CDK1,CCNB1 and Bcl2 were significantly down-regulated(P<0.01).Conclusion Silencing MAPK1 can inhibit the proliferation,invasion and migration of human gastric cancer cells,promote apoptosis and arrest the cell cycle at G1 phase.