文献类型：期刊文献

英文题名：Intervention effect of pinelliae decoction for purging stomach-fire on malignant transformation of bone marrow mesenchymal stem cells in the gastric cancer microenvironment

作者：Liu, Xi-Ping[1];Ming, Hai-Xia[1];Li, Pei-Qing[1]

第一作者：刘喜平

通信作者：Liu, XP[1]

机构：[1]Gansu Univ Chinese Med, Basic Med Coll, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Basic Med Coll, Lanzhou 730000, Peoples R China.|[107351d2d02a88e1f325f]甘肃中医药大学基础医学院（敦煌医学研究所）;[10735]甘肃中医药大学;

年份：2016

卷号：8

期号：7

起止页码：2937

外文期刊名：AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH

收录：;Scopus(收录号：2-s2.0-84979895655);WOS:【SCI-EXPANDED(收录号:WOS:000381647300007)】；

基金：This work was supported by the National Natural Science Foundation of China (812-60525).

语种：英文

外文关键词：Pinelliae decoction; purging stomach-fire; gastric cancer; bone marrow mesenchymal stem cells

摘要：Objective: The study aimed to simulate the microenvironment of gastric cancer to promote the malignant transformation of bone marrow mesenchymal stem cells (BMSCs) and further evaluate the effect of Pinelliae Decoction for Purging Stomach-Fire and its disassembled prescriptions on BMSCs. Methods: Transwell co-culture was performed on the human gastric cancer cell strains BGC-823 and BMSCs to simulate the microenvironment of gastric cancer. The drug-containing serum prepared by Pinelliae Decoction for Purging Stomach-Fire and its disassembled prescriptions was used, and its influence on BMSCs with malignant transformation was observed. Results: BMSCs were harvested successfully from the rat bone marrow, and flow cytometer identification indicated that CD44+/CD34-cells accounted for 70.64%. The co-culture of BGC-823 cells can induce malignant transformation of BMSCs. And the drug-containing serum can induce G2 phase arrest, inhibit cell proliferation, simultaneously inhibit TERT and c-myc expression, lower the cellular ability of chemotactic migration, inhibit the tumor-forming ability of BGC-823 in nude rats and promote the tumor apoptosis. Conclusion: The effective components of Pinelliae Decoction for Purging Stomach-Fire in gastric cancer treatment are pinelliae and dried ginger, and the main acting mechanism is to inhibit tumor cell proliferation and chemotactic migration and promote apoptosis.