文献类型：期刊文献

英文题名：A NIR dual-channel fluorescent probe for the detection of cyanide in living cells

作者：Xie, Yong-Qiang[1];Suo, Qiang[1];Jiang, Shan[1];Li, Lin[1];Ren, Chun-Yan[1];Wu, Rong[1];Shi, Xiao-Ning[1];Zhao, Xiao-Qiang[1];Wang, Li-Na[1];Yin, Reng-Jie[2];Chen, Hui[1];Zhang, Hao-Bo[1]

第一作者：谢永强

通信作者：Xie, YQ[1];Chen, H[1];Zhang, HB[1]

机构：[1]Gansu Univ Chinese Med, Coll Pharm, Expt & Training Teaching Ctr, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Univ Chinese Med, Sch Publ Hlth, Lanzhou 730000, Gansu, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Expt & Training Teaching Ctr, Lanzhou 730000, Gansu, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2025

外文期刊名：PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES

收录：;EI(收录号：20252518635192);Scopus(收录号：2-s2.0-105008343377);WOS:【SCI-EXPANDED(收录号:WOS:001511365900001)】；

基金：This work was supported by the Natural Science Foundation of Gansu Province (23JRRA1214); Higher Education Innovative Foundation of Gansu Province (No. 2024A-96,2020A-077, 2022A-066, 2023B-103); Youth Foundation of Gansu University of Chinese Medicine (ZQ2017-10); Gansu Provincial University Young Doctor Support Program (No. 2025QB-065);Gansu University of Chinese Medicine Science Research and Innovation Fund (No. 2023KCZD-5).

语种：英文

外文关键词：NIR; Triphenylamine; Hemicyanine; Cyanide detection; Fluorescent probe

摘要：This study details the design and synthesis of a near-infrared (NIR) dual-channel cyanide detection fluorescent probe based on triphenylamine-hemicyanine. TPA-CY successfully utilizes the NIR fluorescence properties of triphenylamine-hemicyanine conjugates, showcasing good selectivity and sensitivity, facilitating the visual monitoring of cyanide anions in living cells. Interaction between TPA-CY and CN- occurs rapidly, within a mere 25 s. The sensitivity of TPA-CY to cyanide ions is good, with detection thresholds reaching 0.60 mu M and 0.66 mu M for UV and fluorescence spectra, respectively. The detection mechanism of this probe is the nucleophilic addition of CN- to the C = N bond, which hindered intramolecular charge transfer. Moreover, the probe's efficacy in tracking cyanide radicals in biological systems is validated through live cell imaging.