文献类型：期刊文献

中文题名：甘肃产藏药五脉绿绒蒿有效部位对肝纤维化大鼠炎症相关因子的影响

英文题名：Influence of the active fractions of Meconopsis quintuplinervia Regel growing in Gansu region on inflammatory cytokines in experimental liver fibrosis rat

作者：王志旺[1,2];邵晶[1,3];郭玫[1,3];王瑞琼[1];任远[1,2]

第一作者：王志旺

机构：[1]甘肃中医学院药学系,兰州730000;[2]甘肃省中药药理与毒理学重点实验室;[3]甘肃省高校中(藏)药化学与质量研究省级重点实验室

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2013

卷号：29

期号：1

起止页码：24

中文期刊名：免疫学杂志

外文期刊名：Immunological Journal

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：甘肃省中医药英才基金项目(GZK-2010-52);甘肃省中医药管理局基金项目(GZK-2008-31)

语种：中文

中文关键词：肝纤维化;四氯化碳;五脉绿绒蒿;总黄酮;细胞因子

外文关键词：Hepatic fibrosis; Carbon tetrachloride; Meconopsis quintuplinervia Regel; The total flavones; Cytokines

摘要：目的观察甘肃产藏药五脉绿绒蒿有效部位对肝纤维化大鼠炎症相关因子的影响。方法在给药的基础上,采用皮下注射CCl4、喂饲高脂低蛋白复合饲料并饮用20%乙醇来复制肝纤维化动物模型,6周后对大鼠肝组织HE染色,做病理组织学观察,检测血清酶与相关细胞因子水平,对甘肃产藏药五脉绿绒蒿各提取部位防治肝纤维化疗效进行评价。结果复合因素肝纤维化造模结束后,模型对照组大鼠肝组织病理结果显示其肝纤维化处于Ⅱ-Ⅲ期;与模型对照组比较,甘肃产藏药五脉绿绒蒿醇提物、黄碱组以及总黄酮能明显降低血清ALT、AST与TNF-α、IL-6,显著升高IFN-γ,改善纤维化大鼠细胞因子水平失衡状态,同时发现总黄酮的作用有一定的量效关系。结论甘肃产藏药五脉绿绒蒿醇提物及其中的总黄酮对CCl4复合因素诱导的肝纤维化大鼠血清细胞因子水平失衡有一定的改善作用。
To investigate the influence of active fractions of Meconopsis quintuplinervia Regel （MQR） growing in Gansu province on related cytokines of experimental liver fibrosis in rat. By subcutaneous injection of CCl4, feeding high-fat low-protein diet and drinking 20% alcohol for 6 weeks, the model of hepatic fibrosis rats was established to evaluate the effects of the extractive parts from MQR through detecting serum cytokines and observing hepatic pathology. We found that the ethanol extractive, total flavones, and the mixture of total flavones and total alkaloids from MQR growing in Gansu province could significantly decrease ALT, AST, TNF-α, and IL-6 in serum, but increase IFN-γ and improve disequilibrium of cytokines in experimental liver fibrosis rat. Furthermore, the effects of total flavones exhibited dose-effect relationship. All the result suggested that the ethanol extractive and total flavones of MQR growing in Gansu province have better balancing effects on disequilibrium of cytokines of liver fibrosis in rats induced by CCl4 composited factor.