文献类型：期刊文献

中文题名：大孔吸附树脂分离纯化锁阳总黄酮工艺研究

英文题名：Study of separation and purification of total flavonoids from Cynomorium songaricum Rupr. with macroporous adsorption resin

作者：张君菡[1,2,3];刘永峰[2,3];裴栋[2,3];刘毅[2,3];魏鉴腾[2,3];杨勇智[4];邸多隆[1,2,3]

第一作者：张君菡

机构：[1]甘肃中医药大学药学院,甘肃兰州730000;[2]中国科学院兰州化学物理研究所中国科学院西北特色植物资源化学重点实验室和甘肃天然药物重点实验室,甘肃兰州730000;[3]中国科学院兰州化学物理研究所青岛市资源化学与新材料研究中心,山东青岛266100;[4]阿拉善盟万铭生物制品有限公司,内蒙古阿拉善盟750300

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2015

卷号：26

期号：10

起止页码：2349

中文期刊名：时珍国医国药

外文期刊名：Lishizhen Medicine and Materia Medica Research

收录：北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：国家高技术研究发展计划("863"计划)(2014AA022203);国家自然科学基金(No.21374128);内蒙古阿拉善盟(中科院)沙生资源植物产业项目

语种：中文

中文关键词：大孔吸附树脂;锁阳;总黄酮;工艺研究

外文关键词：Macroporous adsorption resin; Cynomorium songaricum Rupr.; Total flavonoids; Technique

摘要：目的建立大孔吸附树脂分离纯化锁阳总黄酮工艺。方法以大孔吸附树脂对锁阳总黄酮的分离效率和转移率为评价指标,通过静态和动态吸附/解吸附实验优化工艺条件。结果考察了6种大孔吸附树脂对锁阳总黄酮的分离纯化性能,以吸附/解吸附性能为评价指标,确定了GS-6为最佳大孔吸附树脂。在GS-6大孔吸附树脂上优选的最佳工艺条件为:上样浓度为0.038 g·ml-1(相当于原生药),以6 BV·h-1流速吸附,上样量为45 BV(树脂床体积),4 BV水冲洗树脂柱,8 BV的70%乙醇以4 BV·h-1的流速解吸附。在优化的工艺条件下,锁阳总黄酮得率为82.85%,纯度为83.44%。结论优选的GS-6大孔吸附树脂对锁阳总黄酮吸附容量、解吸附率和含量结果良好,优化的生产工艺条件适用于工业化生产。
Objective To establish the separation and purification process of the total flavonoids from Cynomorium songaricum Rupr. by macroporous adsorption resin（ MAR）. Methods The optimal conditions of separation and purification of total flavonoids were obtained based on separation efficiency and transfer ratio of the total flavonoids of static and dynamic adsorption / desorption experiments. Results The separation and purification of the total flavonoids on the six MARs were investigated. The results showed GS- 6 exhibited highest adsorption / desorption properties and was optimal. The optimum process condition on GS- 6 was obtained as follows： for the adsorption process,the volume of feeding samples,concentration,flow rate were 45 BV（ bed volume）,0. 038 g·ml- 1（ equivalent to raw material） and 6 BV·h- 1,respectively; for the desorption process,total flavonoids-loaded MAR column was first washed by 4 BV water（ flow rate： 6 BV·h- 1）,then desorbed by 8 BV ethanol- aqueous solution（ 70： 30,v/v）（ flow rate： 4 BV·h- 1）. Under the optimal conditions,the discovery ratio of the total flavonoids and purity were82. 85% and 83. 44%,respectively. Conclusion GS- 6 exhibited higher adsorption capacity,desorption efficiency and purification efficiency for the total flavonoids. The optimal technique may be used to produce the total flavonoids on a large scale.