文献类型：期刊文献

英文题名：An improved reverse genetics system for rotavirus vaccine strain LLR using five plasmid vectors

作者：Liu, Xiafei[1,2];Yu, Junjie[2,3];Li, Shan[2,3];Chai, Pengdi[2];Xie, Zhiping[2];Pang, Lili[2];Li, Jinsong[2];Zhu, Wuyang[2];Ren, Weihong[4];Duan, Zhaojun[2]

第一作者：Liu, Xiafei

通信作者：Duan, ZJ[1];Ren, WH[2]

机构：[1]Henan Univ Chinese Med, Clin Med Inst 1, Zhengzhou 450000, Henan, Peoples R China;[2]Natl Key Natl Inst Viral Dis Control & Prevent, Beijing 100052, Peoples R China;[3]Gansu Univ Chinese Med, Coll Publ Hlth, Lanzhou 730000, Gansu, Peoples R China;[4]Henan Univ Chinese Med, Affiliated Hosp 1, Zhengzhou 450000, Henan, Peoples R China

第一机构：Henan Univ Chinese Med, Clin Med Inst 1, Zhengzhou 450000, Henan, Peoples R China

通信机构：[1]corresponding author), Natl Key Natl Inst Viral Dis Control & Prevent, Beijing 100052, Peoples R China;[2]corresponding author), Henan Univ Chinese Med, Affiliated Hosp 1, Zhengzhou 450000, Henan, Peoples R China.

年份：2025

卷号：106

期号：1

外文期刊名：JOURNAL OF GENERAL VIROLOGY

收录：;Scopus(收录号：2-s2.0-85214910890);WOS:【SCI-EXPANDED(收录号:WOS:001421022500002)】；

基金：Funding information This work was supported by the National Natural Science Foundation of China (grant no. 82402625) and the Postdoctoral Fellowship Programme (Grade B) of China Postdoctoral Science Foundation (grant no. GZB20240207) .

语种：英文

外文关键词：five- plasmid reverse genetics system; LLR; NLuc; recombinant; rotavirus

摘要：Species A rotaviruses (RVs), which belong to the family Sedoreoviridae and contain a genome of 11 segmented dsRNA segments, are a leading cause of severe acute gastroenteritis in infants and children younger than 5 years of age. We previously developed a strategy to recover rotavirus vaccine strain LLR from 11 cloned plasmids. Here, we report an improved reverse genetics system for LLR by combining two or three transcriptional cassettes in a single plasmid, which substantially enhances rescue efficiency from 66.7% (8/12) to 91.7 % (11/12). Furthermore, the recombinant LLR stably expressing NLuc was rescued based on the five- plasmid reverse genetics system. Improvements to the rotavirus reverse genetics system will enhance its applicability for studies of rotavirus biology and clinical use.