文献类型：期刊文献

英文题名：Whole-genome identification of LdELF4s and analysis of their expression response to diurnal temperature variations in Lilium davidii var. willmottiae (E. H. Wilson) raffill

作者：Ma, Xiaohui[1,2];Li, Ying[3];Guo, Xudong[1,2];Meng, Lingyu[1];Wang, Yinquan[1,2];Su, Junji[3];Jin, Ling[1,2]

第一作者：马晓辉

通信作者：Ma, XH[1];Jin, L[1];Ma, XH[2];Jin, L[2]

机构：[1]Gansu Univ Chinese Med, Lanzhou, Gansu, Peoples R China;[2]Gansu Univ Chinese Med, Gansu Pharmaceut Ind Innovat Res Inst, Lanzhou, Gansu, Peoples R China;[3]Gansu Agr Univ, Coll Life Sci & Technol, State Key Lab Aridland Crop Sci, Lanzhou, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Lanzhou, Gansu, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Gansu Pharmaceut Ind Innovat Res Inst, Lanzhou, Gansu, Peoples R China.|[10735]甘肃中医药大学;

年份：2026

卷号：16

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;Scopus(收录号：2-s2.0-105027960383);WOS:【SCI-EXPANDED(收录号:WOS:001665903300001)】；

基金：The author(s) declared financial support was received for this work and/or its publication. This work was supported by the earmarked fund for CARS-21 and Northwest Collaborative Innovation Center for Traditional Chinese Medicine Co. constructed by Gansu Province & MOE of the PRC.

语种：英文

外文关键词：Lilium davidii var. willmottiae; diurnal temperature variation; ELF4 gene family; genome-wide identification; gene expression

摘要：Introduction Lanzhou lily (Lilium davidii var. willmottiae), a medicinal and edible plant endemic to China, synthesizes bioactive compounds regulated by diurnal temperature variations (DIFs). ELF4 family genes, key regulators coordinating endogenous rhythms with external changes, are unexplored in this species.Methods We analyzed nuclear-cytoplasmic phylogeny across 11 species and identified ELF4 homologs. Phylogenetic, collinearity, cis-element, and expression pattern analyses were conducted for Lanzhou lily ELF4s (LdELF4s). qRT-PCR assessed LdELF4 expression under different DIFs (20/20 degrees C, 25/10 degrees C, 20/5 degrees C day/night), and corresponding phenotypes were evaluated.Results Cytonuclear discordance was observed within Lilium. 62 ELF4 homologs were identified and classified into four subfamilies. Six LdELF4s were located on chromosomes LG01, LG08, LG09, LG12, showing structural variation compared to Lilium sargentiae. LdELF4.1, LdELF4.3, and LdELF4.6 were significantly upregulated at 25/10 degrees C on day 14, while LdELF4.4 and LdELF4.5 were downregulated at 20/5 degrees C. LdELF4.6 showed highest sensitivity. Phenotypically, 25/10 degrees C and 20/5 degrees C treatments delayed flowering by 12.5 and 7.8 days, respectively. The 25/10 degrees C treatment increased stem diameter but reduced flower bud number.Discussion/Conclusion LdELF4.6 is a core candidate gene regulating DIF response in Lanzhou lily, with 25/10 degrees C potentially optimal for bulb development. This study provides genetic resources for breeding climate-adaptive lilies.