文献类型：期刊文献

中文题名：少腹逐瘀汤对子宫内膜异位症大鼠MAPK/ERK信号通路的影响

英文题名：Effect of Shaofu-Zhuyu decoction on MAPK/ERK signaling pathway in rats with endometriosis

作者：王金霞[1];崔宇红[1];成映霞[1];范琳琳[1];张小花[1];范东英[1]

第一作者：王金霞

机构：[1]甘肃中医药大学,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2019

卷号：35

期号：1

起止页码：181

中文期刊名：中国病理生理杂志

外文期刊名：Chinese Journal of Pathophysiology

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：甘肃省自然科学基金资助项目(No.1508RJZA020)

语种：中文

中文关键词：少腹逐瘀汤;子宫内膜异位症;MAPK/ERK信号通路

外文关键词：Shaofu-Zhuyu decoction;Endometriosis;MAPK/ERK signaling pathway

摘要：目的:观察少腹逐瘀汤对子宫内膜异位症大鼠丝裂原激活蛋白激酶(MAPK)/细胞外信号调节激酶(ERK)信号通路的影响,探讨少腹逐瘀汤治疗子宫内膜异位症的分子机制。方法:健康雌性SD大鼠随机分为空白对照组、模型组、痛经宝阳性对照组及少腹逐瘀汤低、中、高剂量组。构建大鼠子宫内腹异位症模型,药物处理4周后,HE染色观察异位子宫组织的病理变化; ELISA法检测宫腔组织中肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)及IL-8的含量; RT-qPCR检测宫腔组织中ERK、血管内皮生长因子(VEGF)和基质金属蛋白酶9(MMP-9)的mRNA表达量; Western blot检测宫腔组织中核因子-κB(NF-κB)、MAPK和MAPK-ERK激酶(MEK)的蛋白含量。结果:与模型组相比,少腹逐瘀汤中、高剂量组大鼠子宫组织中TNF-α、IL-6及IL-8含量显著降低(P <0. 05),ERK、VEGF和MMP-9的m RNA表达量显著降低(P <0. 05),NF-κB、MEK和MAPK的蛋白表达显著下降(P <0. 05)。结论:少腹逐瘀汤通过调节MAPK/ERK信号通路中关键分子的作用,达到治疗子宫内膜异位症的效果。
AIM: To observe the effects of Shaofu-Zhuyu decoction( SFZY) on mitogen-activated protein kinase( MAPK)/extracellular signal-regulated kinase( ERK) signaling pathway in the rats with endometriosis( EM),and to explore the mechanism of SFZY for treatment of EM. METHODS: Healthy female SD rats were used to establish the EM model. The rats were randomly divided into blank control group,model group,positive control group,and low dose,middle dose and high dose of SFZY groups. The pathological changes of the endometriotic tissue were observed by HE staining.The levels of tumor necrosis factor-α( TNF-α),interleukin-6( IL-6) and IL-8 in the uterine tissue were detected by ELISA. The m RNA expression of ERK,vascular endothelial growh factor( VEGF) and matrix metalloprotein-9( MMP-9)was detected by RT-qPCR. The protein expression of nuclear factor-κB( NF-κB),MAPK and MAPK-ERK kinase( MEK)was determined by Western blot. RESULTS: Compared with model group,the levels of TNF-α,IL-6 and IL-8 in the uterine tissue of the rats in middle dose and high dose of SFZY groups were significantly decreased( P < 0. 05),the m RNA expression of ERK,VEGF and MMP-9 was significantly reduced,and the protein expression of NF-κB,MEK and MAPK was decreased significantly in the rat endometriotic tissues( P < 0. 05). CONCLUSION: SFZY may play a key role in the treatment of EM by regulating MAPK/ERK signaling pathway.