文献类型：期刊文献

中文题名：慈菇消脂丸对NF-κB介导的非酒精性脂肪肝病大鼠肝细胞凋亡的干预作用

英文题名：Effects of Cigu Xiaozhi Wan on NF-κB mediated nonalcoholic fatty liver apoptosis

作者：马燕花[1];杨少军[2];白洲霞[3];徐三鹏[1]

第一作者：马燕花

机构：[1]甘肃中医药大学,甘肃730000;[2]广西北海市中医医院;[3]甘肃中医药大学附属医院

第一机构：甘肃中医药大学

年份：2017

卷号：40

期号：10

起止页码：856

中文期刊名：北京中医药大学学报

外文期刊名：Journal of Beijing University of Traditional Chinese Medicine

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金地区科学基金项目(No.81460710)~~

语种：中文

中文关键词：慈菇消脂丸;非酒精性脂肪肝;细胞凋亡;核转录因子κB;B细胞淋巴瘤-2;Bcl-2相关X蛋白;大鼠

外文关键词：Sagittaria lipid-reducing Pill; nonalcoholic fatty liver disease ; apoptosis ; NF-κB ; Bcl-2 ;Bax ; rats

摘要：目的通过观察慈菇消脂丸对高脂饮食诱导非酒精性脂肪肝病(NAFLD)大鼠肝细胞凋亡相关调控基因核转录因子κB(NF-κB)、B细胞淋巴瘤-2基因(Bcl-2)、Bcl-2相关X蛋白(Bax)表达的影响,探讨解毒化痰法治疗NAFLD的作用机制。方法 60只SPF级健康雄性Wistar大鼠,随机分为6组:正常对照组,模型组,慈菇消脂丸高、中、低剂量组,盐酸吡格列酮组,每组10只。采用高脂饮食喂饲大鼠建立NAFLD模型后,连续药物治疗6周。给药结束后,处死全部动物,取肝脏和血清。全自动生化分析仪检测各组大鼠肝功、血脂;HE染色观察肝脏的组织形态学特点;透射电子显微镜观察肝细胞的超微结构;Western Blot技术检测肝组织NF-κB、Bcl-2、Bax的表达。结果与正常组比较,模型组大鼠血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、甘油三酯(TG)、胆固醇(TC)、低密度脂蛋白(LDL-C)水平升高(P<0.01);肝组织呈中重度脂肪变性并同时伴有肝细胞凋亡;肝组织中NF-κB、Bax蛋白表达增强(P<0.01),Bcl-2蛋白表达减低(P<0.01),Bcl-2/Bax比值降低(P<0.01)。与模型组比较,慈菇消脂丸各剂量组大鼠血清ALT、AST、TG、TC水平降低(P<0.01);肝组织NF-κB、Bax蛋白表达减低(P<0.01),Bcl-2蛋白和Bcl-2/Bax比值升高(P<0.05)。与盐酸吡格列酮组比较,慈菇消脂丸高剂量下调NF-κB蛋白、上调Bcl-2蛋白表达的作用更显著(P<0.01)。结论慈菇消脂丸能够明显改善NAFLD大鼠肝功及血脂;上调Bcl-2蛋白表达,降低NF-κB、Bax蛋白表达,使Bcl-2/Bax比值升高,从而抑制肝细胞凋亡,起到防治NAFLD的作用。
ObjectiveTo study the effects of Cigu Xiaozhi Wan（Sagittaria Lipid-reducing Pill,CGXZW）on the expression of nuclear factor-κgene binding（NF-κB）,B-cell lymphana-2（Bcl-2）and Bcl-2 associated X protein（Bax）in rats with nonalcoholic fatty liver disease（NAFLD）and explore the therapeutic mechanism of toxin-removing phlegm-resolving method.MethodsSPF grade male Wistar rats（n=60）were randomly divided into six groups：normal control group,model group,low-dose,middose and high-dose CGXZW groups,and pioglitazone hydrochloride group（n=10 in each group）.All rats were fed with high-fat diet to establish NAFLD model except those in the normal control group.After6 weeks of intervention,all the experimental animals were sacrificed.Liver and serum sample were taken.Serum samples were drawn to test liver function and blood lipid by automatic biochemical analyzer.The liver histopathological changes were observed under microscope with HE staining.The ultrastructure of liver cells was observed by using transmission electron microscope.The expression of NF-κB,Bcl-2 and Bax in rats of all groups was detected by using Western blot.Results Compared with the normal group,the serum levels of alanine aminotransferase（ALT）,aspartate transaminase（AST）,triglyceride（TG）,total cholesterol（TC）and low density lipoprotein-cholesterol（LDL-C）were significantly increased（P〈0.01）in the model group;liver tissue of NAFLD rats showed moderate to severe steatosis and hepatocytes apoptosis.The expression of NF-κB and Bax in liver tissue of rats were increased significantly（P〈0.01）,while the expression of Bcl-2 and Bcl-2/Bax ratio were significantly reduced（P〈0.01）in the model group.Compared with the model group,the serum levels of ALT,AST,TG,TC and LDL-C were decreased significantly in all-dose CGXZW groups（P〈0.01）;the expression of NF-κB and Bax were significantly reduced（P〈0.01）whereas the expression of Bcl-2 and the ratio of Bcl-2/Bax was increased（P〈0.05）.CGXZW high-dose group was superior to pioglitazone hydrochloride group in terms of down-regulation of NF-κB and up-regulation the expression of Bcl-2（P〈0.01）whereas pioglitazone hydrochloride group was better than all CGXZW groups in down-regulating the expression of Bax and up-regulating the ratio of Bcl-2/Bax（P〈0.01）.Conclusion Sagittaria Lipid-reducing Pill can significantly improve liver function and reduce blood lipid level in NAFLD rats,upregulate the expression of Bcl-2,decrease the expression of NF-κB and Bax,and inhibit liver cell apoptosis.It could be used in the prevention and treatment of NAFLD.