文献类型：期刊文献

英文题名：Human umbilical cord mesenchymal stem cell-derived exosomes mitigate acute radiation-induced intestinal oxidative damage via the Nrf2/HO-1/NQO1 signaling pathway

作者：Wang, Hongyu[1,2];Wang, Jinbao[1,2];Yang, Gaosheng[1,2];Li, Yanjie[1,2];Chen, Weikai[2];Yu, Jianping[2];Han, Xiaopeng[2]

第一作者：汪海燕;Wang, Hongyu

通信作者：Yu, JP[1];Han, XP[1]

机构：[1]Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou, Peoples R China;[2]Gansu Prov Cent Hosp, Dept Gen Surg 1, Lanzhou, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Prov Cent Hosp, Dept Gen Surg 1, Lanzhou, Peoples R China.

年份：2025

卷号：20

期号：6

外文期刊名：PLOS ONE

收录：;Scopus(收录号：2-s2.0-105007853390);WOS:【SCI-EXPANDED(收录号:WOS:001504294500027)】；

基金：I would like to express my gratitude to the General Surgery Department I of Gansu Provincial Central Hospital for their support and encouragement, as well as to Ms. JJW for her invaluable assistance.All authors declare that they have not use AI-generated work in this manuscript.

语种：英文

摘要：Acute radiation-induced intestinal injury (ARII), a prevalent complication of abdominal radiotherapy, remains clinically challenging due to limited therapeutic options. This study demonstrates the therapeutic efficacy of human umbilical cord mesenchymal stem cell-derived exosomes (hucMSC-Exos) in mitigating ARII through Nrf2/HO-1/NQO1 pathway activation. In a rat model receiving 12 Gy abdominal irradiation, systemic hucMSC-Exos administration significantly restored intestinal mucosal integrity and reduced oxidative damage markers. Mechanistically, hucMSC-Exos potentiated the antioxidant axis by upregulating Nrf2 signaling, as evidenced by histopathological, biochemical, and molecular analyses. Complementary in vitro experiments revealed hucMSC-Exos protected irradiated IEC-6 cells from oxidative dysfunction while enhancing proliferation, effects substantially attenuated upon Nrf2 silencing via siRNA. These findings establish that hucMSC-Exos orchestrate redox equilibrium through targeted Nrf2 pathway modulation, effectively counteracting radiation-induced enterocyte apoptosis. The elucidated mechanism expands the therapeutic paradigm of MSC-derived exosomes in radioprotection and provides a clinically translatable strategy for managing ARII in oncological radiotherapy.