文献类型：期刊文献

英文题名：Phytoestrogen genistein decreases contractile response of aortic artery in vitro and arterial blood pressure in vivo

作者：Li H.-F.[1];Wang L.-D.[2];Qu S.-Y.[1]

第一作者：Li H.-F.

通信作者：Li, HF[1]

机构：[1]Lanzhou Med Coll, Dept Physiol, Lanzhou 730000, Peoples R China;[2]Gansu Chinese Med Coll, Affiliated Hosp, Lanzhou 730020, Peoples R China

第一机构：Lanzhou Med Coll, Dept Physiol, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Lanzhou Med Coll, Dept Physiol, Lanzhou 730000, Peoples R China.

年份：2004

卷号：25

期号：3

起止页码：313

外文期刊名：ACTA PHARMACOLOGICA SINICA

收录：;Scopus(收录号：2-s2.0-1542374054);WOS:【SCI-EXPANDED(收录号:WOS:000220117800011)】；

语种：英文

外文关键词：genistein; aorta; smooth muscle; muscle relaxation; calcium channel; vascular endothelium; blood pressure

摘要：AIM: To determine the mechanisms of effects of phytoestrogen genistein on the contracted rabbit aortic arteries in vitro, and observe the effect of genistein and 17-beta estradiol on mean arterial pressure (MAP) in ovariectomized (OVX) rats. METHODS: (1) Strips of rabbit aortic smooth muscle were suspended in organ baths containing Kreb's solution, and then isometric tension was measured. (2) Female mature Wistar rats underwent a bilateral ovariectomy (OVX). Sham-operated rats (SHAM) were used as controls. After administration of genistein (0.4 mg . kg(-1 .) d(-1), sc), 17-beta estradiol (1 mg . kg(-1) . d(-1), sc) or their vehicle sesame oil for 21 d, MAP was measured. RESULTS: (1) Similar to 17-beta estradiol. genistein could dose-dependently relax 40 mmol/L KCl-precontracted arterial strips. Incubation with N-omega-L-nitro-arginine (L-NNA), methylene blue (MB), indomethacin, propranolol or endothelium removal did not affect relaxation induced by genistein. In calcium-free solution containing 0.01mmol/L egtazic acid (EGTA), genistein inhibited not only the first phase contraction induced by noradrenaline (NA), but also the second contraction induced by CaCl2. In addition, genistem could reduce the contractile responses of NA, KCl and CaCl, and shift their cumulative concentration-response curves rightward. (2) MAP in OVX rats was significantly higher compared with that of SHAM rats. However, after chronically treatment with genistein or 17-beta estradiol for 21 d the baseline MAP in OVX rats was reduced significantly. CONCLUSIONS: (1) The vasodilator effect of genistein in vitro is endothelium independent and not related to the nitric oxide, its mechanisms being probably due to inhibition of Ca2+ influx through calcium channels in a noncompetitive manner and Ca2+ release from intracellular store induced by NA. (2) Administration of genistein or 17-beta estradiol can chronically decrease MAP in OVX rats.