文献类型：期刊文献

英文题名：Global DNA hypomethylation, rather than reactive oxygen species (ROS), a potential facilitator of cadmium-stimulated K562 cell proliferation

作者：Huang, Dejun[1];Zhang, Yingmei[1];Qi, Yongmei[1];Chen, Che[1,2];Ji, Weihong[3]

第一作者：Huang, Dejun

通信作者：Zhang, YM[1]

机构：[1]Lanzhou Univ, Sch Life Sci, Key Lab Arid & Grassland Ecol, Lanzhou 730000, Peoples R China;[2]Gansu Coll Tradit Chinese Med, Lanzhou 730000, Peoples R China;[3]Massey Univ, Inst Nat Resources, Auckland, New Zealand

第一机构：Lanzhou Univ, Sch Life Sci, Key Lab Arid & Grassland Ecol, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Sch Life Sci, Key Lab Arid & Grassland Ecol, 222 Tianshui Rd, Lanzhou 730000, Peoples R China.

年份：2008

卷号：179

期号：1

起止页码：43

外文期刊名：TOXICOLOGY LETTERS

收录：;Scopus(收录号：2-s2.0-44149105575);WOS:【SCI-EXPANDED(收录号:WOS:000257134700007)】；

基金：This work was supported by National Natural Science Foundation of China (No. 30770390) and Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, China (No. XJDX0201-2007-01).

语种：英文

外文关键词：heavy metal; cell proliferation; ROS; DNA methylation; DNA damage; carcinogenesis

摘要：Cell proliferation plays a critical role in the process of cadmium (Cd) carcinogenesis. Although both induction of reactive oxygen species (ROS) and alteration of DNA methylation are involved in Cd-stimulated cell proliferation, the detailed mechanism of Cd-stimulated cell proliferation remains poorly understood. In this study, K562 cells pre-treated with N-acetylcysteine (NAC) or methionine (Meth) were exposed to Cd to investigate the potential contribution of ROS and global DNA methylation pathways in Cd-induced cell proliferation. The results showed that Cd-stimulated cell proliferation, increased ROS and DNA damage levels, and induced global DNA hypomethylation. The increases of ROS and DNA damage levels were attenuated by pre-treatment with NAC. Cd-stimulated cell proliferation did not appear to be suppressed through eliminating ROS by NAC. However, methionine was shown to prevent Cd-induced global DNA hypomethylation and Cd-stimulated cell proliferation. Our results suggest that global DNA hypomethylation, rather than ROS, is a potential facilitator of Cd-stimulated K562 cell proliferation. (c) 2008 Published by Elsevier Ireland Ltd.