文献类型：期刊文献

中文题名：基于“病-证-症-方”关联网络探讨杜仲腰痛丸的功效特点及作用机制

英文题名：Pharmacological Characteristics and Mechanisms of Duzhong Yaotong Pills Based on the“Disease-syndrome-symptom-formula”Association Network

作者：周诗鸿[1];吴依凡[1];张立存[1];陈丹丹[1];修明慧[1];和建政[1,2];赵继荣[1]

第一作者：周诗鸿

机构：[1]甘肃中医药大学,兰州730000;[2]敦煌医学与转化教育部重点实验室,兰州730000

第一机构：甘肃中医药大学

年份：2025

卷号：20

期号：22

起止页码：4024

中文期刊名：世界中医药

外文期刊名：World Chinese Medicine

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金项目(82460940);甘肃省联合科研基金重大项目(23JRRA1519);2024年高等学校人才培养质量提升和创新创业教育改革项目(GSZY-2024-09)。

语种：中文

中文关键词：杜仲腰痛丸;腰椎间盘突出症;“病-证-症-方”关联网络;功效特点;作用机制;神经功能;炎症反应;激素代谢

外文关键词：Duzhong Yaotong Pills;Lumbar disc herniation;“Disease-syndrome-symptom-formula”association network;Pharmacological characteristics;Pharmacological mechanism;Neurological function;Inflammatory response;Hormone metabolism

摘要：目的:基于“病-证-症-方”关联网络分析策略,探析杜仲腰痛丸干预腰椎间盘突出症(LDH)的功效特点和作用机制。方法:根据杜仲腰痛丸组方方解对功效组进行划分;通过网络药理学相关数据库获取杜仲腰痛丸活性成分及相关靶点预测,构建蛋白质互作网络,对关键靶点进行生物富集分析。使用斯泼累格·多雷雄性大鼠进行LDH模型制备;利用转录组学、中医证候本体及多维定量关联计算平台及基因集分析杜仲腰痛丸干预LDH机制;采用实时荧光定量PCR检测信号通路基因mRNA水平变化。结果:网络分析表明,杜仲腰痛丸方中补肾壮腰组、活血消肿组与通络止痛组的药理特点分别概括为神经功能改善、炎症反应调节、激素代谢水平纠正;结合转录组学、基因集富集分析及分子生物学结果,发现杜仲腰痛丸抑制磷脂酰肌醇3-激酶(PI3K)、蛋白激酶B(AKT)基因表达(P<0.01),干预炎症介质释放如白细胞介素-8(IL-8)(P<0.05)、肿瘤坏死因子-α(TNF-α)(P<0.01),通过纠正疾病发生发展中的“神经-免疫-内分泌”失衡系统来干预LDH关键致病基因。结论:杜仲腰痛丸通过调节神经功能、激素水平及改善炎症反应,发挥温肾壮腰、行气止痛的功效,从而缓解LDH疼痛症状。
Objective:Based on the“disease-syndrome-symptom-formula”network analysis strategy,this study aimed to investigate the pharmacological characteristics and mechanisms of Duzhong Yaotong Pills in the treatment of lumbar disc herniation(LDH).Methods:Efficacy groups were categorized according to the formula composition and traditional explanations of Duzhong Yaotong Pills.Active ingredients and related targets were identified using network pharmacology databases,and protein-protein interaction networks were constructed.Key targets were subjected to bioinformatic enrichment analysis.Sprague Dawley male rats were used to establish an LDH model.Transcriptomics,the Traditional Chinese Medicine Syndrome Ontology,multi-dimensional quantitative association analysis platforms,and gene set analysis were applied to explore the mechanisms of Duzhong Yaotong Pills in LDH.Real-time quantitative PCR was used to detect changes in mRNA expression of signaling pathway genes.Results:Network analysis showed that the Bushen Zhuangyao group,Huoxue Xiaozhong group,and Tongluo Zhitong group of Duzhong Yaotong Pills were primarily associated with improving neurological function,regulating inflammatory responses,and correcting hormone metabolism levels,respectively.Integrating transcriptomics,gene set enrichment analysis,and molecular biology results,Duzhong Yaotong Pills were found to inhibit the expression of phosphoinositide 3-kinase(PI3K)and protein kinase B(AKT)genes(P<0.01),modulate the release of inflammatory mediators such as interleukin-8(IL-8)(P<0.05)and tumor necrosis factor-α(TNF-α)(P<0.01),and intervene in key pathogenic genes of LDH by correcting imbalances in the neuro-immune-endocrine system during disease progression.Conclusion:Duzhong Yaotong Pills alleviate LDH-related pain by regulating neurological function,hormone levels,and inflammatory responses,thereby exerting therapeutic effects consistent with warming the kidneys,strengthening the waist,promoting qi circulation,and relieving pain.