文献类型：期刊文献

中文题名：连作对纹党生长、生理特性及自毒物质的影响研究

英文题名：Research on effects of continuous cropping on the growth,physiological characteristics and autotoxic substances of Codonopsis pilosula var.modesta

作者：邱亚娟[1];邵晓龄[1];陈莺宇[1];黄钰芳[1,2,4];陈红刚[1,2];杨扶德[1];高素芳[1,2];袁菊丽[3];高健[5]

第一作者：邱亚娟

机构：[1]甘肃中医药大学药学院,甘肃兰州730000;[2]西北中藏药省部共建协同创新中心,甘肃兰州730000;[3]甘肃中医药大学教学实验实训中心,甘肃兰州730000;[4]敦煌医学与转化教育部重点实验室,甘肃兰州730000;[5]甘肃文县中寨纹党产区代元有限公司,甘肃陇南742500

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2025

卷号：34

期号：1

起止页码：107

中文期刊名：草业学报

外文期刊名：Acta Prataculturae Sinica

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金项目(82160714);甘肃省自然科学基金项目(21JR1RA271);国家重点研发计划项目(2018YFC1706305);甘肃省高等学校产业支撑引导项目(2021CYZC-40);2023年研究生“创新之星”项目(2023CXZX-764);敦煌医学与转化教育部重点实验室项目(DHYZX20-13)资助。

语种：中文

中文关键词：纹党;连作障碍;生理生化;化感自毒作用;气相色谱-质谱分析

外文关键词：Codonopsis pilosula var.modesta;continuous cropping obstacles;physiology and biochemistry;allelopathic autotoxicity;gas chromatography-mass spectrometry

摘要：为探究纹党连作障碍效应及发生机制,以正茬(CK)和连作1年(CC1)、连作2年(CC2)及连作3年(CC3)土壤为试验对象,开展盆栽试验,动态研究连作对纹党生长、抗氧化酶系统及叶绿素的影响,并利用气相色谱-质谱联用技术(GC-MS)分析了不同连作年限土壤中自毒物质的种类及含量。结果显示,苗期、开花期、生长旺盛期及收获期各个生育时期内,连作对纹党的生长均存在抑制作用,且随连作年限的延长作用逐渐加强;同一连作年限,随着生育进程的推进,叶片中过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性先增加后下降,过氧化物酶(POD)活性先下降后增加再下降,但在同一生长阶段,3种抗氧化酶活性均随连作年限增加逐年下降,而丙二醛(MDA)、脯氨酸(Pro)、可溶性糖含量和相对电导率均逐年增加;从CK、CC1、CC2和CC3中分别鉴定出5、17、13和4种化学物质,主要包括:邻苯二甲酸二(2-乙基己)酯、邻苯二甲酸二丁酯、丙酸乙酯和油酸酰胺等物质,其中邻苯二甲酸二(2-乙基己)酯在各种处理土壤中均存在,其相对含量均较高。连作可以通过破坏纹党植株中抗氧化酶系统,降低叶片叶绿素含量抑制纹党生长,产生连作障碍;连作条件下自毒作用是导致纹党发生连作障碍的主要原因之一。
In order to explore the continuous cropping obstacle effect of Codonopsis pilosula var.modesta and its mechanism,pot experiments were conducted in the soil of normal crop(CK),and continuous cropping 1 year(CC1),continuous cropping 2 years(CC2)and continuous cropping 3 years(CC3)as experimental objects.The effects of continuous cropping on the growth,antioxidant enzyme system and chlorophyll of C.pilosula var.modesta were evaluated,and the types and levels of autotoxic substances in the soil of different continuous cropping years were analyzed by gas chromatography-mass spectrometry(GC-MS).The results showed that continuous cropping had an inhibitory effect on the growth of C.pilosula var.modesta at the seedling stage,flowering stage,peak growth stage and harvest stage,and the effect gradually strengthened with increase in the number of continuous cropping years.In the same continuous cropping years,with the advancement of growth process,the activities of catalase(CAT)and superoxide dismutase(SOD)in leaves initially increased and then decreased,and the activities of peroxidase(POD)initially decreased and then increased and then decreased again,but at the same growth stage,the activities of the three antioxidant enzymes all decreased year by year,and but the levels of malondialdehyde(MDA),proline(Pro),soluble sugar and the relative conductivity all increased year by year.The results of GC-MS showed that 5,17,13 and 4 chemical substances were identified from CK,CC1,CC2 and CC3,respectively,including di-2-ethylhexyl phthalate,dibutyl phthalate,ethyl propionate and oleamide,among which di-2-ethylhexyl phthalate was found in all treatments with relatively high levels.It can be seen that continuous cropping can inhibit the growth of C.pilosula var.modesta by disrupting the antioxidant enzyme system and reducing the leaf chlorophyll content,resulting in continuous cropping disorders.The autotoxicity under continuous cropping is one of the main causes for continuous cropping disorders in C.pilosula var.modesta.