文献类型：期刊文献

中文题名：补阳还五汤预处理对心肌缺血再灌注损伤大鼠抗氧化功能的影响

英文题名：Effects of Buyang Huanwu Decoction pretreatment on antioxidant function in rats with myocardial ischemia-reperfusion injury

作者：宋忠阳[1];陈玉婵[2];雍文兴[3];张志明[3];刘永琦[4];张利英[4];张卫强[3]

第一作者：宋忠阳

机构：[1]甘肃中医药大学中医临床学院,兰州730000;[2]甘肃中医药大学定西校区,甘肃定西743000;[3]甘肃中医药大学附属医院,兰州730000;[4]甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,兰州730000

第一机构：甘肃中医药大学中医临床学院

年份：2020

卷号：26

期号：11

起止页码：1621

中文期刊名：中国中医基础医学杂志

外文期刊名：JOURNAL OF BASIC CHINESE MEDICINE

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

基金：国家自然科学基金资助项目(81660730)-益气活血法基于PI3K-AKT通路调控BMSCs心肌MIRI靶向归巢及其自噬与凋亡动态平衡研究;甘肃省重点研发计划(18YF1FA044)-基于16SrRNA基因测序技术燥湿和胃法治疗胃溃疡活动期(脾胃湿热型)患者舌苔微生态及其菌群相关性研究。

语种：中文

中文关键词：补阳还五汤;心肌缺血再灌注损伤;心肌酶;抗氧化功能

外文关键词：Buyang Huanwu Decoction;Myocardial ischemia reperfusion injury;Myocardial enzyme;antioxidant

摘要：目的:研究补阳还五汤预处理对心肌缺血再灌注损伤(MIRI)大鼠心肌酶及抗氧化功能的影响。方法:将40只Wistar大鼠随机分为假手术组(Sham组)、缺血再灌注组(MIRI组)、缺血预适应组、补阳还五汤组各10只。造模前,4组大鼠分别连续灌胃7 d,补阳还五汤组用补阳还五汤灌胃,其余各组用等量生理盐水灌胃,采用结扎冠状动脉左前降支(LAD)方法复制MIRI大鼠模型。通过大鼠心电图评判是否造模成功,采用生化法检测血清乳酸脱氢酶(lactate dehydroge-nase,LDH)和肌酸激酶(creatine kinase,CK)的活性,采用硫代巴比妥酸法测定血清丙二醛(malondialdehyde,MDA)含量,黄嘌呤氧化酶法测定血清超氧化物歧化酶(superoxide dismutase,SOD)活性及硝酸还原酶法测定一氧化氮(Nitric oxide,NO)含量。结果:与MIRI模型组比较,补阳还五汤组血清LDH和CK活性明显降低,MDA含量明显降低,SOD活性及NO含量明显升高。结论:补阳还五汤可提高MIRI大鼠血清中SOD活性,增加NO含量,同时降低LDH、CK活性和MDA含量,减轻MIRI,其机制与减轻细胞膜脂质过氧化反应,防护再灌注期心肌细胞的氧化应激损伤有关。
Objective:To study the effects of Buyang Huanwu Decoction pretreatment on myocardial enzymes and antioxidant functions in myocardial ischemia reperfusion injury(MIRI)rats.Methods:40 Wistar rats were randomly divided into Sham group(Sham group),ischemia reperfusion group(MIRI group),ischemia preconditioning group and Buyang Huanwu Decoction group.Before the modeling,the rats in the four groups were given continuous gavage for 7d,the rats in the Buyang Huanwu Decoction group were given gavage with Buyang Huanwu decoction,and the other groups were given normal saline,and the MIRI rat model was replicated by ligating the left anterior descending coronary artery(LAD)method.ECG was used to evaluate the success of rats modeling,biochemical method was used to detect the serum lactate dehydrogenase(LDH)and creatine kinase(CK)activity.The determination of malondialdehyde(MDA)in serum was measured by thiobarbituric acid method.Serum superoxide dismutase(SOD)activity was measured by xanthine oxidase method.NO content was measured by Nitrate reductase method.Results:compared with the MIRI model group,serum LDH and CK activity in Buyang Huanwu Decoction group decreased significantly.MDA concentration was significantly decreased,SOD activity and NO content were significantly increased.Conclusion:Buyang Huanwu Decoction can increase SOD activity,increase NO content,decrease LDH,CK activity and MDA concentration in serum of MIRI rats,and reduce MIRI.Its mechanism is related to reducing lipid peroxidation reaction of cell membrane and protecting myocardial cells from oxidative stress injury during reperfusion.